Crystal structure of the Ube2K/E2-25K and K48-linked di-ubiquitin complex provides structural insight into the mechanism of K48-specific ubiquitin chain synthesis.
Sign Up to like & getrecommendations! Published in 2018 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2018.10.067
Abstract: Ubiquitin-conjugating enzymes (E2) form thioester bonds with ubiquitin (Ub), which are subsequently transferred to target proteins for cellular progress. Ube2K/E2-25K (a class II E2 enzyme) contains a C-terminal ubiquitin-associated (UBA) domain that has been suggested… read more here.
Keywords: crystal structure; k48; ube2k 25k; k48 linked ... See more keywords
Proteomics Links Ubiquitin Chain Topology Change to Transcription Factor Activation.
Sign Up to like & getrecommendations! Published in 2019 at "Molecular cell"
DOI: 10.1016/j.molcel.2019.07.001
Abstract: A surprising complexity of ubiquitin signaling has emerged with identification of different ubiquitin chain topologies. However, mechanisms of how the diverse ubiquitin codes control biological processes remain poorly understood. Here, we use quantitative whole-proteome mass… read more here.
Keywords: chain; met4; topology; transcription factor ... See more keywords
Ufd2p synthesizes branched ubiquitin chains to promote the degradation of substrates modified with atypical chains
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DOI: 10.1038/ncomms14274
Abstract: Ubiquitination of a subset of proteins by ubiquitin chain elongation factors (E4), represented by Ufd2p in Saccharomyces cerevisiae, is a pivotal regulator for many biological processes. However, the mechanism of Ufd2p-mediated ubiquitination is largely unclear.… read more here.
Keywords: substrates modified; degradation; ubiquitin chain; branched ubiquitin ... See more keywords
Mechanistic insights into the subversion of the linear ubiquitin chain assembly complex by the E3 ligase IpaH1.4 of Shigella flexneri
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2116776119
Abstract: Significance Shigella flexneri, a deleterious bacterium, causes massive human infection cases and deaths worldwide. To facilitate survival and replication in infected host cells, S. flexneri can secrete two highly similar E3 ligase effectors, IpaH1.4 and… read more here.
Keywords: chain assembly; shigella flexneri; ubiquitin chain; assembly complex ... See more keywords