Implementation of ubiquitous chromatin opening elements as artificial integration sites for CRISPR/Cas9‐mediated knock‐in in mammalian cells
Sign Up to like & getrecommendations! Published in 2023 at "Engineering in Life Sciences"
DOI: 10.1002/elsc.202200047
Abstract: CRISPR/Cas9‐mediated targeted gene integration (TI) has been used to generate recombinant mammalian cell lines with predictable transgene expression. Identifying genomic hot spots that render high and stable transgene expression and knock‐in (KI) efficiency is critical… read more here.
Keywords: crispr cas9; ubiquitous chromatin; integration; cas9 ... See more keywords
Ubiquitous Chromatin-opening Elements (UCOEs): Applications in biomanufacturing and gene therapy.
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology advances"
DOI: 10.1016/j.biotechadv.2017.05.004
Abstract: Ubiquitous Chromatin-opening Elements (UCOEs) are defined by their ability to consistently confer stable, site of integration-independent transgene expression that is proportional to copy number, including from within regions of heterochromatin such as centromeres. UCOEs structurally… read more here.
Keywords: opening elements; ubiquitous chromatin; chromatin opening; gene ... See more keywords
The CpG-sites of the CBX3 ubiquitous chromatin opening element are critical structural determinants for the anti-silencing function
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DOI: 10.1038/s41598-017-04212-8
Abstract: Suppression of therapeutic transgene expression from retroviral gene therapy vectors by epigenetic defence mechanisms represents a problem that is particularly encountered in pluripotent stem cells (PSCs) and their differentiated progeny. Transgene expression in these cells,… read more here.
Keywords: cpg sites; ubiquitous chromatin; anti silencing; chromatin opening ... See more keywords