Establishment of a five-enzyme cell-free cascade for the synthesis of uridine diphosphate N-acetylglucosamine.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of biotechnology"
DOI: 10.1016/j.jbiotec.2018.07.027
Abstract: In spite of huge endeavors in cell line engineering to produce glycoproteins with desired and uniform glycoforms, it is still not possible in vivo. Alternatively, in vitro glycoengineering can be used for the modification of… read more here.
Keywords: glcnac; acetylglucosamine; cell free; enzyme ... See more keywords
GFPT2/GFAT2 and AMDHD2 act in tandem to control the hexosamine pathway
Sign Up to like & getrecommendations! Published in 2022 at "eLife"
DOI: 10.7554/elife.69223
Abstract: The hexosamine biosynthetic pathway (HBP) produces the essential metabolite UDP-GlcNAc and plays a key role in metabolism, health, and aging. The HBP is controlled by its rate-limiting enzyme glutamine fructose-6-phosphate amidotransferase (GFPT/GFAT) that is directly… read more here.
Keywords: udp glcnac; gfpt2; amdhd2; hbp ... See more keywords