Ultrahigh-Throughput Screening of Metagenomic Libraries Using Droplet Microfluidics.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1826-4_2
Abstract: Droplet microfluidics enables the ultrahigh-throughput screening of the natural or man-made genetic diversity for industrial enzymes, with reduced reagent consumption and lower costs than conventional robotic alternatives. Here we describe an example of metagenomic screening… read more here.
Keywords: ultrahigh throughput; screening metagenomic; droplet microfluidics; throughput screening ... See more keywords
Speeding up enzyme discovery and engineering with ultrahigh-throughput methods.
Sign Up to like & getrecommendations! Published in 2018 at "Current opinion in structural biology"
DOI: 10.1016/j.sbi.2017.12.010
Abstract: Exploring the sequence space of enzyme catalysts is ultimately a numbers game. Ultrahigh-throughput screening methods for rapid analysis of millions of variants are therefore increasingly important for investigating sequence-function relationships, searching large metagenomic libraries for… read more here.
Keywords: discovery engineering; throughput; enzyme discovery; ultrahigh throughput ... See more keywords
Ultrahigh-Throughput Enzyme Engineering and Discovery in In Vitro Compartments
Sign Up to like & getrecommendations! Published in 2023 at "Chemical Reviews"
DOI: 10.1021/acs.chemrev.2c00910
Abstract: Novel and improved biocatalysts are increasingly sourced from libraries via experimental screening. The success of such campaigns is crucially dependent on the number of candidates tested. Water-in-oil emulsion droplets can replace the classical test tube,… read more here.
Keywords: vitro compartments; droplet; ultrahigh throughput; droplet screening ... See more keywords
Ultrahigh-Throughput Production of Monodisperse and Multifunctional Janus Microparticles Using in-Air Microfluidics
Sign Up to like & getrecommendations! Published in 2018 at "ACS Applied Materials & Interfaces"
DOI: 10.1021/acsami.8b05227
Abstract: Compartmentalized Janus microparticles advance many applications ranging from chemical synthesis to consumer electronics. Although these particles can be accurately manufactured using microfluidic droplet generators, the per-nozzle throughputs are relatively low (∼μL/min). Here, we use “in-air… read more here.
Keywords: production; janus microparticles; air microfluidics; ultrahigh throughput ... See more keywords
Cell-free Directed Evolution of a Protease in Microdroplets at Ultrahigh Throughput
Sign Up to like & getrecommendations! Published in 2021 at "ACS Synthetic Biology"
DOI: 10.1021/acssynbio.0c00538
Abstract: Compartmentalization of single genes in water-in-oil emulsion droplets is a powerful approach to create millions of reactors for enzyme library selections. When these droplets are formed at ultrahigh throughput in microfluidic devices, their perfect monodispersity… read more here.
Keywords: free directed; evolution; protease; cell free ... See more keywords
Functional Deimmunization of Botulinum Neurotoxin Protease Domain via Computationally Driven Library Design and Ultrahigh-Throughput Screening.
Sign Up to like & getrecommendations! Published in 2023 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.2c00426
Abstract: Botulinum neurotoxin serotype A (BoNT/A) is a widely used cosmetic agent that also has diverse therapeutic applications; however, adverse antidrug immune responses and associated loss of efficacy have been reported in clinical uses. Here, we… read more here.
Keywords: botulinum neurotoxin; throughput screening; ultrahigh throughput; design ultrahigh ... See more keywords
Abseq: Ultrahigh-throughput single cell protein profiling with droplet microfluidic barcoding
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/srep44447
Abstract: Proteins are the primary effectors of cellular function, including cellular metabolism, structural dynamics, and information processing. However, quantitative characterization of proteins at the single-cell level is challenging due to the tiny amount of protein available.… read more here.
Keywords: single cell; protein; ultrahigh throughput; cell ... See more keywords
Ultrahigh-throughput functional profiling of microbiota communities
Sign Up to like & getrecommendations! Published in 2018 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.1811250115
Abstract: Significance Analyzing complex microbial communities is the milestone of modern microbiology, calling for “deep functional profiling” techniques. While next generation sequencing revolutionized our understanding of microbiota communities, we still lack high-throughput technologies to precisely determine… read more here.
Keywords: microbiota; functional profiling; microbiota communities; microbiology ... See more keywords
Optimization of Ultrahigh-Throughput Screening Assay for Protein Engineering of d-Allulose 3-Epimerase
Sign Up to like & getrecommendations! Published in 2022 at "Biomolecules"
DOI: 10.3390/biom12111547
Abstract: d-Allulose is the corresponding epimer of d-fructose at the C-3 position, which exhibits a similar taste and sweetness to sucrose. As a low-calorie sweetener, d-allulose has broad application prospects in the fields of medicine, food,… read more here.
Keywords: daease; screening assay; allulose epimerase; throughput screening ... See more keywords
Ultrahigh-Throughput Screening of High-β-Xylosidase-Producing Penicillium piceum and Investigation of the Novel β-Xylosidase Characteristics
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Fungi"
DOI: 10.3390/jof8040325
Abstract: A droplet-based microfluidic ultrahigh-throughput screening technology has been developed for the selection of high-β-xylosidase-producing Penicillium piceum W6 from the atmospheric and room-temperature plasma-mutated library of P. piceum. β-xylosidase hyperproducers filamentous fungi, P. piceum W6, exhibited… read more here.
Keywords: xylosidase producing; throughput screening; ultrahigh throughput; xylosidase ... See more keywords