3D Probed Lipid Dynamics in Small Unilamellar Vesicles.
Sign Up to like & getrecommendations! Published in 2017 at "Small"
DOI: 10.1002/smll.201603408
Abstract: Single-molecule fluorescence correlation spectroscopy overcomes the resolution barrier of optical microscopy (10≈-20 nm) and is utilized to look into lipid dynamics in small unilamellar vesicles (SUVs; diameter < 100 nm). The fluorescence trajectories of lipid-like… read more here.
Keywords: lipid dynamics; small unilamellar; unilamellar vesicles; dynamics small ... See more keywords
Magainin-H2 effects on the permeabilization and mechanical properties of giant unilamellar vesicles.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of colloid and interface science"
DOI: 10.1016/j.jcis.2019.06.028
Abstract: Among the potential novel therapeutics to treat bacterial infections, antimicrobial peptides (AMPs) are a very promising substitute due to their broad-spectrum activity and rapid bactericidal action. AMPs strongly interact with the bacterial membrane, and the… read more here.
Keywords: lipid bilayers; mag; magainin effects; mechanical properties ... See more keywords
Multicomponent and Multiphase Lipid Nanotubes Formed by Gliding Microtubule-Kinesin Motility and Phase-Separated Giant Unilamellar Vesicles
Sign Up to like & getrecommendations! Published in 2019 at "Langmuir"
DOI: 10.1021/acs.langmuir.9b02637
Abstract: Cytoskeletal filaments and motor proteins are critical components in the transport and reorganization of membrane-based organelles in eukaryotic cells. Previous studies have recapitulated the microtubule-kinesin transport system in vitro to dynamically assemble large-scale nanotube networks… read more here.
Keywords: microtubule kinesin; unilamellar vesicles; phase separated; phase ... See more keywords
Wrapping Pathways of Anisotropic Dumbbell Particles by Giant Unilamellar Vesicles
Sign Up to like & getrecommendations! Published in 2023 at "Nano Letters"
DOI: 10.1021/acs.nanolett.3c00375
Abstract: Endocytosis is a key cellular process involved in the uptake of nutrients, pathogens, or the therapy of diseases. Most studies have focused on spherical objects, whereas biologically relevant shapes can be highly anisotropic. In this… read more here.
Keywords: unilamellar vesicles; giant unilamellar; anisotropic dumbbell; wrapping pathways ... See more keywords
DisGUVery: A Versatile Open-Source Software for High-Throughput Image Analysis of Giant Unilamellar Vesicles
Sign Up to like & getrecommendations! Published in 2022 at "ACS Synthetic Biology"
DOI: 10.1021/acssynbio.2c00407
Abstract: Giant Unilamellar Vesicles (GUVs) are cell-sized aqueous compartments enclosed by a phospholipid bilayer. Due to their cell-mimicking properties, GUVs have become a widespread experimental tool in synthetic biology to study membrane properties and cellular processes.… read more here.
Keywords: unilamellar vesicles; microscopy; analysis; giant unilamellar ... See more keywords
Control of Enzyme Reaction Initiation inside Giant Unilamellar Vesicles by the Cell-Penetrating Peptide-Mediated Translocation of Cargo Proteins.
Sign Up to like & getrecommendations! Published in 2022 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.2c00413
Abstract: Cell-penetrating peptides (CPPs) play important roles in directly delivering biomolecules, such as DNA, proteins, and peptides, into living cells. In artificial lipid membranes, such as planar lipid bilayers, the direct membrane translocation of β-galactosidase via… read more here.
Keywords: translocation; unilamellar vesicles; guvs; microscopy ... See more keywords
Imaging of photoacoustic-mediated permeabilization of giant unilamellar vesicles (GUVs)
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-82140-4
Abstract: Target delivery of large foreign materials to cells requires transient permeabilization of the cell membrane without toxicity. Giant unilamellar vesicles (GUVs) mimic the phospholipid bilayer of the cell membrane and are also useful drug delivery… read more here.
Keywords: vesicles guvs; permeabilization; giant unilamellar; unilamellar vesicles ... See more keywords
Production of giant unilamellar vesicles and encapsulation of lyotropic nematic liquid crystals.
Sign Up to like & getrecommendations! Published in 2021 at "Soft matter"
DOI: 10.1039/d0sm01684e
Abstract: We describe a modified microfluidic method for making Giant Unilamellar Vesicles (GUVs) via water/octanol-lipid/water double emulsion droplets. At a high enough lipid concentration we show that the de-wetting of the octanol from these droplets occurs… read more here.
Keywords: nematic liquid; liquid; liquid crystals; lyotropic nematic ... See more keywords
Assessing membrane material properties from the response of giant unilamellar vesicles to electric fields.
Sign Up to like & getrecommendations! Published in 2022 at "Advances in physics: X"
DOI: 10.1080/23746149.2022.2125342
Abstract: Knowledge of the material properties of membranes is crucial to understanding cell viability and physiology. A number of methods have been developed to probe membranes in vitro, utilizing the response of minimal biomimetic membrane models… read more here.
Keywords: membrane; unilamellar vesicles; material properties; giant unilamellar ... See more keywords
Systematic comparison of unilamellar vesicles reveals that archaeal core lipid membranes are more permeable than bacterial membranes
Sign Up to like & getrecommendations! Published in 2023 at "PLOS Biology"
DOI: 10.1371/journal.pbio.3002048
Abstract: One of the deepest branches in the tree of life separates the Archaea from the Bacteria. These prokaryotic groups have distinct cellular systems including fundamentally different phospholipid membrane bilayers. This dichotomy has been termed the… read more here.
Keywords: core; unilamellar vesicles; bacterial membranes; membrane permeability ... See more keywords
Integrin-Functionalised Giant Unilamellar Vesicles via Gel-Assisted Formation: Good Practices and Pitfalls
Sign Up to like & getrecommendations! Published in 2021 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms22126335
Abstract: Giant unilamellar vesicles (GUV) are powerful tools to explore physics and biochemistry of the cell membrane in controlled conditions. For example, GUVs were extensively used to probe cell adhesion, but often using non-physiological linkers, due… read more here.
Keywords: gel assisted; formation; unilamellar vesicles; giant unilamellar ... See more keywords