Articles with "universal hairpin" as a keyword



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OUHP: an optimized universal hairpin primer system for cost-effective and high-throughput RT-qPCR-based quantification of microRNA (miRNA) expression

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Published in 2021 at "Nucleic Acids Research"

DOI: 10.1093/nar/gkab1153

Abstract: Abstract MicroRNAs (miRNAs or miRs) are single-stranded, ∼22-nucleotide noncoding RNAs that regulate many cellular processes. While numerous miRNA quantification technologies are available, a recent analysis of 12 commercial platforms revealed high variations in reproducibility, sensitivity,… read more here.

Keywords: mirna quantification; system; quantification; universal hairpin ... See more keywords