Comprehensive single-PCR 16S and 18S rRNA community analysis validated with mock communities, and estimation of sequencing bias against 18S.
Sign Up to like & getrecommendations! Published in 2021 at "Environmental microbiology"
DOI: 10.1111/1462-2920.15553
Abstract: Universal primers for SSU rRNA genes allow profiling of natural communities by simultaneously amplifying templates from Bacteria, Archaea, and Eukaryota in a single PCR reaction. Despite the potential to show relative abundance for all rRNA… read more here.
Keywords: rrna; mock; universal primers; pcr ... See more keywords
Human Alphacoronavirus Universal Primers for Genome Amplification and Sequencing
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2022.789665
Abstract: Rapid and accurate sequencing covering the entire genome is essential to identify genetic variations of viral pathogens. However, due to the low viral titers in clinical samples, certain amplification steps are required for viral genome… read more here.
Keywords: entire genome; genome amplification; universal primers; primer set ... See more keywords