Photo-Crosslinkable Unnatural Amino Acids Enable Facile Synthesis of Thermoresponsive Nano- to Microgels of Intrinsically Disordered Polypeptides.
Sign Up to like & getrecommendations! Published in 2018 at "Advanced materials"
DOI: 10.1002/adma.201704878
Abstract: Hydrogel particles are versatile materials that provide exquisite, tunable control over the sequestration and delivery of materials in pharmaceutics, tissue engineering, and photonics. The favorable properties of hydrogel particles depend largely on their size, and… read more here.
Keywords: crosslinkable unnatural; size; photo crosslinkable; unnatural amino ... See more keywords
In vitro suppression of two different stop codons
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26226
Abstract: Proteins play a crucial role in all living organisms, with the 20 natural amino acids as their building blocks. Unnatural amino acids are synthetic derivatives of these natural building blocks. These amino acids have unique… read more here.
Keywords: amino acids; different stop; two different; unnatural amino ... See more keywords
Linkage‐Specific Synthesis of Di‐ubiquitin Probes Enabled by the Incorporation of Unnatural Amino Acid ThzK
Sign Up to like & getrecommendations! Published in 2022 at "ChemBioChem"
DOI: 10.1002/cbic.202200133
Abstract: Di‐ubiquitin (diUB) conjugates of defined linkages are useful tools for probing the functions of UB ligases, UB‐binding proteins and deubiquitinating enzymes (DUBs) in coding, decoding and editing the signals carried by the UB chains. Here… read more here.
Keywords: incorporation unnatural; linkage; specific synthesis; unnatural amino ... See more keywords
Unnatural amino acids improve affinity and modulate immunogenicity: Developing peptides to treat MHC type II autoimmune disorders
Sign Up to like & getrecommendations! Published in 2019 at "Peptide Science"
DOI: 10.1002/pep2.24058
Abstract: Many autoimmune diseases, including multiple sclerosis (MS), rheumatoid arthritis (RA), and celiac disease (CD), arise from improper immune system recognition of self or benign peptides as threats. No autoimmune disease currently has a cure. Many… read more here.
Keywords: amino; amino acids; response; antigen ... See more keywords
Synthesis of Unnatural α-Amino Acid Derivatives via Photoredox Activation of Inert C(sp3)-H Bonds.
Sign Up to like & getrecommendations! Published in 2022 at "Organic letters"
DOI: 10.1021/acs.orglett.2c01822
Abstract: The synthesis of unnatural, tertiary amino acids is a challenging task. While decarboxylation-radical addition has been an important strategy for their formation, the use of alkyl radicals from C(sp3)-H bonds has not been fully explored.… read more here.
Keywords: synthesis unnatural; sp3 bonds; amino acid; unnatural amino ... See more keywords
Fluorescent Probes with Unnatural Amino Acids to Monitor Proteasome Activity in Real-Time.
Sign Up to like & getrecommendations! Published in 2020 at "ACS chemical biology"
DOI: 10.1021/acschembio.0c00634
Abstract: The proteasome is an essential protein complex that, when dysregulated, can result in various diseases in eukaryotic cells. As such, understanding the enzymatic activity of the proteasome and what can alter it is crucial to… read more here.
Keywords: amino acids; proteasome activity; unnatural amino; activity ... See more keywords
Unraveling the Photoactivation Mechanism of a Light-Activated Adenylyl Cyclase Using Ultrafast Spectroscopy Coupled with Unnatural Amino Acid Mutagenesis
Sign Up to like & getrecommendations! Published in 2022 at "ACS Chemical Biology"
DOI: 10.1021/acschembio.2c00575
Abstract: The hydrogen bonding network that surrounds the flavin in blue light using flavin adenine dinucleotide (BLUF) photoreceptors plays a crucial role in sensing and communicating the changes in the electronic structure of the flavin to… read more here.
Keywords: spectroscopy; photoactivation; adenylyl cyclase; unnatural amino ... See more keywords
Combining Sense and Nonsense Codon Reassignment for Site-Selective Protein Modification with Unnatural Amino Acids.
Sign Up to like & getrecommendations! Published in 2017 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.6b00245
Abstract: Incorporation of unnatural amino acids (uAAs) via codon reassignment is a powerful approach for introducing novel chemical and biological properties to synthesized polypeptides. However, the site-selective incorporation of multiple uAAs into polypeptides is hampered by… read more here.
Keywords: amino acids; site selective; sense; unnatural amino ... See more keywords
Amino Acid Schiff Base Bearing Benzophenone Imine As a Platform for Highly Congested Unnatural α-Amino Acid Synthesis.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.0c02707
Abstract: Unnatural α-amino acids are invaluable building blocks in synthetic organic chemistry and could upgrade the function of peptides. We developed a new mode for catalytic activation of amino acid Schiff bases, serving as a platform… read more here.
Keywords: highly congested; amino; amino acid; acid schiff ... See more keywords
Post-translational mutagenesis for installation of natural and unnatural amino acid side chains into recombinant proteins
Sign Up to like & getrecommendations! Published in 2017 at "Nature Protocols"
DOI: 10.1038/nprot.2017.087
Abstract: Methods for installing natural and unnatural amino acids and their modifications into proteins in a benign and precise manner are highly sought-after in protein science. Here we describe a protocol for 'post-translational mutagenesis' that enables… read more here.
Keywords: mutagenesis; side chains; post translational; natural unnatural ... See more keywords
Azobenzene-based unnatural amino acid scaffolds via a Pd(II)-catalyzed C(sp3)-H arylation strategy.
Sign Up to like & getrecommendations! Published in 2022 at "Chemical communications"
DOI: 10.1039/d2cc04870a
Abstract: Azobenzene-based unnatural amino acid motifs were synthesized via Pd(II)-catalyzed diastereoselective C(sp3)-H arylation of amino acid carboxamides with iodoacetanilides and Mills azo coupling. read more here.
Keywords: based unnatural; azobenzene based; amino acid; unnatural amino ... See more keywords