A modular two yeast species secretion system for the production and preparative application of unspecific peroxygenases
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DOI: 10.1038/s42003-021-02076-3
Abstract: Fungal unspecific peroxygenases (UPOs) represent an enzyme class catalysing versatile oxyfunctionalisation reactions on a broad substrate scope. They are occurring as secreted, glycosylated proteins bearing a haem-thiolate active site and rely on hydrogen peroxide as… read more here.
Keywords: secretion; secretion system; production; unspecific peroxygenases ... See more keywords
Broadening the Biocatalytic Toolbox—Screening and Expression of New Unspecific Peroxygenases
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DOI: 10.3390/antiox11020223
Abstract: Unspecific peroxygenases (UPOs) catalyze the selective transfer of single oxygen atoms from peroxides to a broad range of substrates such as un-activated hydrocarbons. Since specific oxyfunctionalizations are among the most-desired reactions in synthetic chemistry, UPOs… read more here.
Keywords: biocatalytic toolbox; expression; unspecific peroxygenases; broadening biocatalytic ... See more keywords
Cell-Free Protein Synthesis with Fungal Lysates for the Rapid Production of Unspecific Peroxygenases
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DOI: 10.3390/antiox11020284
Abstract: Unspecific peroxygenases (UPOs, EC 1.11.2.1) are fungal biocatalysts that have attracted considerable interest for application in chemical syntheses due to their ability to selectively incorporate peroxide-oxygen into non-activated hydrocarbons. However, the number of available and… read more here.
Keywords: unspecific peroxygenases; cell free; protein synthesis; free protein ... See more keywords
Structural Characterization of Two Short Unspecific Peroxygenases: Two Different Dimeric Arrangements
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DOI: 10.3390/antiox11050891
Abstract: Unspecific peroxygenases (UPOs) are extracellular fungal enzymes of biotechnological interest as self-sufficient (and more stable) counterparts of cytochrome P450 monooxygenases, the latter being present in most living cells. Expression hosts and structural information are crucial… read more here.
Keywords: different dimeric; unspecific peroxygenases; characterization two; dimeric arrangements ... See more keywords