Analysis of Nuclear Uracil DNA-Glycosylase (nUDG) Turnover During the Cell Cycle.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6603-5_11
Abstract: Uracil-DNA glycosylases (UDG/UNG) are enzymes that remove uracil from DNA and initiate base-excision repair. These enzymes play a key role in maintaining genomic integrity by reducing the mutagenic events caused by G:C to A:T transition… read more here.
Keywords: uracil dna; dna; cell cycle; dna glycosylase ... See more keywords
Use of a molecular beacon based fluorescent method for assaying uracil DNA glycosylase (Ung) activity and inhibitor screening
Sign Up to like & getrecommendations! Published in 2021 at "Biochemistry and Biophysics Reports"
DOI: 10.1016/j.bbrep.2021.100954
Abstract: Uracil DNA glycosylases are an important class of enzymes that hydrolyze the N-glycosidic bond between the uracil base and the deoxyribose sugar to initiate uracil excision repair. Uracil may arise in DNA either because of… read more here.
Keywords: use molecular; uracil dna; activity; dna glycosylase ... See more keywords
An effective human uracil-DNA glycosylase inhibitor targets the open pre-catalytic active site conformation.
Sign Up to like & getrecommendations! Published in 2021 at "Progress in biophysics and molecular biology"
DOI: 10.1016/j.pbiomolbio.2021.02.004
Abstract: Human uracil DNA-glycosylase (UDG) is the prototypic and first identified DNA glycosylase with a vital role in removing deaminated cytosine and incorporated uracil and 5-fluorouracil (5-FU) from DNA. UDG depletion sensitizes cells to high APOBEC3B… read more here.
Keywords: uracil dna; inhibitor; dna; dna glycosylase ... See more keywords
Single-Nanoparticle ICP-MS for Sensitive Detection of Uracil-DNA Glycosylase Activity.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.1c01447
Abstract: Single-nanoparticle inductively coupled plasma mass spectrometry (SP-ICP-MS) has demonstrated unique advantages for the detection of biological samples. However, methods for enzyme activity detection based on SP-ICP-MS technology have been rarely explored. Here we report the… read more here.
Keywords: icp; detection; uracil dna; single nanoparticle ... See more keywords
Construction of an Entropy-Driven Dumbbell-Type DNAzyme Assembly Circuit for Lighting Up Uracil-DNA Glycosylase in Living Cells.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c03223
Abstract: Sensitive monitoring of intracellular uracil-DNA glycosylase (UDG) in living cells is essential to understanding the DNA repair pathways and discovery of anticancer drugs. Herein, we demonstrate the construction of an entropy-driven dumbbell-type DNAzyme assembly circuit… read more here.
Keywords: dna; entropy driven; dnazyme; uracil dna ... See more keywords
QM/MM Study of the Uracil DNA Glycosylase Reaction Mechanism: A Competition between Asp145 and His148.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of chemical theory and computation"
DOI: 10.1021/acs.jctc.8b01305
Abstract: Uracil DNA glycosylase catalyzes the N-glycosidic bond cleavage of uracil, thereby initiating the base excision repair mechanism for this DNA lesion. Here we employ hybrid quantum mechanics/molecular mechanics calculations to investigate the exact mechanism of… read more here.
Keywords: uracil dna; mechanism; dna; dna glycosylase ... See more keywords
Distance-Based Biosensor for Ultrasensitive Detection of Uracil-DNA Glycosylase Using Membrane Filtration of DNA Hydrogel.
Sign Up to like & getrecommendations! Published in 2021 at "ACS sensors"
DOI: 10.1021/acssensors.1c00624
Abstract: In the deoxyribonucleic acid (DNA) repair pathways, DNA repair enzymes have great significance for genomic integrity. As one important initiator of the base-excision repair pathway, the aberrant activity of uracil-DNA glycosylase (UDG) is closely associated… read more here.
Keywords: uracil dna; detection; dna; distance ... See more keywords
Excision of uracil from DNA by hSMUG1 includes strand incision and processing
Sign Up to like & getrecommendations! Published in 2019 at "Nucleic Acids Research"
DOI: 10.1093/nar/gky1184
Abstract: Abstract Uracil arises in DNA by hydrolytic deamination of cytosine (C) and by erroneous incorporation of deoxyuridine monophosphate opposite adenine, where the former event is devastating by generation of C → thymine transitions. The base… read more here.
Keywords: uracil dna; strand incision; dna; excision ... See more keywords
A structurally conserved motif in γ-herpesvirus uracil-DNA glycosylases elicits duplex nucleotide-flipping
Sign Up to like & getrecommendations! Published in 2018 at "Nucleic Acids Research"
DOI: 10.1093/nar/gky217
Abstract: Abstract Efficient γ-herpesvirus lytic phase replication requires a virally encoded UNG-type uracil-DNA glycosylase as a structural element of the viral replisome. Uniquely, γ-herpesvirus UNGs carry a seven or eight residue insertion of variable sequence in… read more here.
Keywords: uracil dna; duplex nucleotide; dna; loop ... See more keywords
Oxidative Stress-Mediated Overexpression of Uracil DNA Glycosylase in Leishmania donovani Confers Tolerance against Antileishmanial Drugs
Sign Up to like & getrecommendations! Published in 2018 at "Oxidative Medicine and Cellular Longevity"
DOI: 10.1155/2018/4074357
Abstract: Leishmania donovani is an intracellular protozoan parasite that causes endemic tropical disease visceral leishmaniasis (VL). Present drugs used against this fatal disease are facing resistance and toxicity issues. Survival of leishmania inside the host cells… read more here.
Keywords: uracil dna; leishmania donovani; donovani; dna glycosylase ... See more keywords
Abstract 3802: A novel uracil-DNA glycosylase, UdgX, as a new biochemical tool to directly detect uracils in DNA
Sign Up to like & getrecommendations! Published in 2017 at "Cancer Research"
DOI: 10.1158/1538-7445.am2017-3802
Abstract: The enzyme activation-induced deaminase (AID) is essential to the B lymphocyte development and antibody maturation. It converts cytosines in DNA to uracil. The unregulated expression of AID in many B cell cancers is correlated with… read more here.
Keywords: uracil dna; dna; dna glycosylase; cancer ... See more keywords