Determining Protein Phosphorylation Status Using Antibody Arrays and Phos-Tag Biotin.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1064-0_18
Abstract: We describe here a standard protocol for determining the phosphorylation status of protein multiplexes using antibody arrays and a biotinylated Phos-tag with a dodeca(ethylene glycol) spacer (Phos-tag Biotin). The procedure is based on an antibody… read more here.
Keywords: phos tag; phosphorylation status; using antibody;
Aptamer fluorescence anisotropy assays for detection of aflatoxin B1 and adenosine triphosphate using antibody to amplify signal change
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DOI: 10.1039/d2ra00843b
Abstract: Fluorescence polarization/anisotropy (FP/FA) is an attractive technology for determining small molecules in homogeneous solution based on rotation changes of a fluorescent reporter. Binding induced conformation change is a specific property of aptamers. This property has… read more here.
Keywords: change; aptamer; adenosine triphosphate; fluorescence ... See more keywords
High‐throughput analysis of amniotic fluid proteins associated with histological chorioamnionitis in preterm premature rupture of membranes using an antibody‐based microarray
Sign Up to like & getrecommendations! Published in 2022 at "American Journal of Reproductive Immunology"
DOI: 10.1111/aji.13595
Abstract: To identify potential proteins in the amniotic fluid (AF) that may be associated with histologic chorioamnionitis (HCA) in patients with preterm premature rupture of membranes (PPROM) using antibody‐based microarray analysis. read more here.
Keywords: rupture membranes; amniotic fluid; antibody based; premature rupture ... See more keywords