Facile Site-Directed Mutagenesis of Large Constructs Using Gibson Isothermal DNA Assembly.
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DOI: 10.1007/978-1-4939-6472-7_24
Abstract: Site-directed mutagenesis is a commonly used molecular biology technique to manipulate biological sequences, and is especially useful for studying sequence determinants of enzyme function or designing proteins with improved activity. We describe a strategy using… read more here.
Keywords: using gibson; biology; site directed; directed mutagenesis ... See more keywords