Labeling Antibodies Using a Maleimido Dye.
Sign Up to like & getrecommendations! Published in 2019 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot099291
Abstract: Fluorophore-maleimide derivatives are effective for labeling sulfhydryl-containing molecules. Maleimide groups react with free thiols at pH 6.5-7.5 forming a covalent bond. Reducing agents should be avoided during the conjugation step. This protocol uses the cross-linker… read more here.
Keywords: antibodies using; using maleimido; labeling antibodies; maleimido dye ... See more keywords