A High-Throughput Method to Prioritize PROTAC Intracellular Target Engagement and Cell Permeability Using NanoBRET.
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DOI: 10.1007/978-1-0716-1665-9_14
Abstract: Target engagement and cell permeation are important parameters that may limit the efficacy of proteolysis-targeting chimeras (PROTACs). Here, we present an approach that facilitates both the quantitation of PROTAC binding affinity for an E3 ligase… read more here.
Keywords: engagement cell; target engagement; target; using nanobret ... See more keywords