Rapid Pathogen Detection in Infectious Uveitis Using Nanopore Metagenomic Next-Generation Sequencing: A Preliminary Study.
Sign Up to like & getrecommendations! Published in 2023 at "Ocular immunology and inflammation"
DOI: 10.1080/09273948.2023.2173243
Abstract: PURPOSE We establish an accurate and rapid diagnostic method for pathogen detection in infectious uveitis using nanopore metagenomic next-generation sequencing (NGS). METHODS In eight patients with suspected infectious uveitis, we prospectively compared the accuracy and… read more here.
Keywords: nanopore metagenomic; pathogen detection; using nanopore; infectious uveitis ... See more keywords
A workflow for accurate metabarcoding using nanopore MinION sequencing
Sign Up to like & getrecommendations! Published in 2021 at "Methods in Ecology and Evolution"
DOI: 10.1111/2041-210x.13561
Abstract: Metabarcoding has become a common approach to the rapid identification of the species composition in a mixed sample. The majority of studies use established short‐read high‐throughput sequencing platforms. The Oxford Nanopore MinIONTM, a portable sequencing… read more here.
Keywords: consensus; workflow accurate; nanopore minion; accurate metabarcoding ... See more keywords
Profiling of circular RNA N6 -methyladenosine in moso bamboo (Phyllostachys edulis) using nanopore-based direct RNA sequencing.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of integrative plant biology"
DOI: 10.1111/jipb.13002
Abstract: N6 -methyladenosine (m6 A) is a prevalent modification in messenger RNAs and circular RNAs that plays important roles in regulating various aspects of RNA metabolism. However, the occurrence of the m6 A modification in plant… read more here.
Keywords: circular rnas; using nanopore; based direct; rna ... See more keywords
Rapid diagnosis of intra-amniotic infection using nanopore-based sequencing
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Perinatal Medicine"
DOI: 10.1515/jpm-2022-0504
Abstract: Abstract Objectives Early diagnosis and treatment of intra-amniotic infection is crucial. Rapid pathogen identification allows for a definite diagnosis and enables proper management. We determined whether the 16S amplicon sequencing performed by a nanopore sequencing… read more here.
Keywords: amniotic infection; intra amniotic; diagnosis; using nanopore ... See more keywords
Detection and Validation of Circular DNA Fragments Using Nanopore Sequencing
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Genetics"
DOI: 10.3389/fgene.2022.867018
Abstract: Occurrence of extra-chromosomal circular DNA is a phenomenon frequently observed in tumor cells, and the presence of such DNA has been recognized as a marker of adverse outcome across cancer types. We here describe a… read more here.
Keywords: circular dna; dna; dna fragments; detection ... See more keywords
Genetic Surveillance of Five SARS-CoV-2 Clinical Samples in Henan Province Using Nanopore Sequencing
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Immunology"
DOI: 10.3389/fimmu.2022.814806
Abstract: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has rapidly spread and poses a major threat to public health worldwide. The whole genome sequencing plays a crucial role in virus surveillance and evolutionary analysis. In this… read more here.
Keywords: surveillance five; genetic surveillance; using nanopore; nanopore sequencing ... See more keywords