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Using overlap‐extension PCR technique to fusing genes for constructing recombinant plasmids

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0 Published in 2018 at "Journal of Basic Microbiology"

DOI: 10.1002/jobm.201700455

Abstract: Overlap‐extension PCR is a method for splice of gene segments to produce focused fragments for constructing recombinant plasmid, but its complexity limits its application. To simplify the protocol and to improve the effectiveness, we employed… read more here.

Keywords: constructing recombinant; overlap extension; using overlap; extension pcr ... See more keywords