Olivar: towards automated variant aware primer design for multiplex tiled amplicon sequencing of pathogens
Sign Up to like & getrecommendations! Published in 2024 at "Nature Communications"
DOI: 10.1038/s41467-024-49957-9
Abstract: Tiled amplicon sequencing has served as an essential tool for tracking the spread and evolution of pathogens. Over 15 million complete SARS-CoV-2 genomes are now publicly available, most sequenced and assembled via tiled amplicon sequencing.… read more here.
Keywords: primer; automated variant; amplicon sequencing; variant aware ... See more keywords
VARSCOT: variant-aware detection and scoring enables sensitive and personalized off-target detection for CRISPR-Cas9
Sign Up to like & getrecommendations! Published in 2019 at "BMC Biotechnology"
DOI: 10.1186/s12896-019-0535-5
Abstract: BackgroundNatural variations in a genome can drastically alter the CRISPR-Cas9 off-target landscape by creating or removing sites. Despite the resulting potential side-effects from such unaccounted for sites, current off-target detection pipelines are not equipped to… read more here.
Keywords: detection; crispr cas9; variant aware; varscot ... See more keywords