Vector-free in vivo trans-determination of adult hepatic stem cells to insulin-producing cells
Sign Up to like & getrecommendations! Published in 2019 at "Molecular Biology Reports"
DOI: 10.1007/s11033-019-04870-z
Abstract: A reduction in the number of functional β-cells is the central pathological event in diabetes. Liver and ventral pancreas differentiates simultaneously in the same general domain of cells within embryonic endoderm. In addition, the precursor… read more here.
Keywords: vector free; hepatic stem; producing cells; stem ... See more keywords
An enhanced vector-free allele exchange (VFAE) mutagenesis protocol for genome editing in a wide range of bacterial species
Sign Up to like & getrecommendations! Published in 2017 at "AMB Express"
DOI: 10.1186/s13568-017-0425-y
Abstract: Vector-free allele exchange (VFAE) is a newly developed protocol for genome editing in Pseudomonas species. Although several parameters have been determined to optimize the procedures for obtaining a stable and high-frequency mutation, numerous false-positive clones… read more here.
Keywords: genome editing; vector free; allele exchange; protocol ... See more keywords