Vip3Aa domain IV and V mutants confer higher insecticidal activity against Spodoptera frugiperda and Helicoverpa armigera.
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DOI: 10.1002/ps.6858
Abstract: BACKGROUND The fall armyworm Spodoptera frugiperda and cotton bollworm Helicoverpa armigera are major insect pests of corn and cotton worldwide. Genetically engineered crops producing Vip3Aa, a potent endotoxin, from the bacterium Bacillus thuringiensis (Bt) are… read more here.
Keywords: insecticidal activity; vip3aa; activity; armigera ... See more keywords
Utilization of a strong promoter combined with the knockout of protease genes to improve the yield of Vip3Aa in Bacillus thuringiensis BMB171.
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DOI: 10.1002/ps.7343
Abstract: BACKGROUND Vip3Aa is an insecticidal protein secreted by some Bacillus thuringiensis strains during vegetative growth. It has excellent insecticidal activity, its mechanism of action is different from that of Cry protein, and it can delay… read more here.
Keywords: bacillus thuringiensis; protease genes; thuringiensis; vip3aa ... See more keywords
RNA interference of PHB1 enhances virulence of Vip3Aa to Sf9 cells and Spodoptera frugiperda larvae.
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DOI: 10.1002/ps.7469
Abstract: BACKGROUND In our previous work, we demonstrated that prohibitin 2 (PHB2) on the membrane of Sf9 cells was a receptor for Vip3Aa, and PHB2 in mitochondria contributed to the mitochondrial stability to reduce Vip3Aa toxicity.… read more here.
Keywords: phb1; frugiperda larvae; sf9 cells; virulence ... See more keywords
Intracellular Localization and Cytotoxicity of Bacillus thuringiensis Vip3Aa against Spodoptera frugiperda (Sf9) Cells.
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DOI: 10.1016/j.jip.2020.107340
Abstract: Vip3Aa protein is produced by Bacillus thuringiensis during vegetative growth and displays high toxicity against a wide range of lepidopteran insect larvae such as Spodoptera exigua and Spodoptera frugiperda, both important insect pests worldwide. Vip3Aa… read more here.
Keywords: spodoptera frugiperda; intracellular localization; actvip3aa; vip3aa ... See more keywords
Vip3Aa from Bacillus thuringiensis subsp. kurstaki HD1 is toxic to Aedes aegypti (Diptera: Culicidae).
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DOI: 10.1016/j.jip.2020.107342
Abstract: Bacillus thuringensis (Bt) vegetative insecticidal proteins share no sequence or structural homology with the known Cry proteins. The activity of Vip3Aa is generally considered to be restricted to lepidopteran insects. In this paper, the vip3Aa… read more here.
Keywords: aedes aegypti; subsp kurstaki; toxic aedes; vip3aa ... See more keywords
Antifungal Mechanism of Vip3Aa, a Vegetative Insecticidal Protein, against Pathogenic Fungal Strains
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DOI: 10.3390/antibiotics10121558
Abstract: Discovering new antifungal agents is difficult, since, unlike bacteria, mammalian and fungal cells are both eukaryotes. An efficient strategy is to consider new antimicrobial proteins that have variety of action mechanisms. In this study, a… read more here.
Keywords: vip3aa protein; vegetative insecticidal; insecticidal protein; vip3aa ... See more keywords
Resistance Allele Frequency of Helicoverpa zea to Vip3Aa Bacillus thuringiensis Protein in the Southeastern U.S.
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DOI: 10.3390/insects14020161
Abstract: Simple Summary Evolution of insect resistance is the main threat to the sustainability of the Bt technology. Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) is a major target pest of Bt corn and cotton. The purpose of… read more here.
Keywords: resistance allele; frequency; zea; resistance ... See more keywords
Oligomer Formation and Insecticidal Activity of Bacillus thuringiensis Vip3Aa Toxin
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DOI: 10.3390/toxins12040274
Abstract: Bacillus thuringiensis (Bt) Vip3A proteins are important insecticidal proteins used for control of lepidopteran insects. However, the mode of action of Vip3A toxin is still unclear. In this study, the amino acid residue S164 in… read more here.
Keywords: 240 kda; formation; kda complex; toxin ... See more keywords
Vegetative Insecticidal Protein Vip3Aa Is Transported via Membrane Vesicles in Bacillus thuringiensis BMB171
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DOI: 10.3390/toxins14070480
Abstract: Vegetative insecticidal protein Vip3Aa, secreted by many Bacillus thuringiensis (Bt) strains during the vegetative growth stage, represents the second-generation insecticidal toxin. In recent years, significant progress has been made on its structure and action mechanism.… read more here.
Keywords: vegetative insecticidal; vip3aa; thuringiensis; protein vip3aa ... See more keywords