Viral Delivery of GFP-Dependent Recombinases to the Mouse Brain.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7169-5_8
Abstract: Many genetic tools have been developed that use green fluorescent protein (GFP) and its derivatives for labeling specific cell populations in organisms and in cell culture. To extend the use of GFP beyond labeling purposes,… read more here.
Keywords: mouse brain; gfp dependent; gfp; viral delivery ... See more keywords
Stable Expression of Epigenome Editors via Viral Delivery and Genomic Integration.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7774-1_11
Abstract: The advent of precise genomic targeting systems has revolutionized epigenome editing through fusion of epigenetic effector proteins with engineered DNA-binding proteins. However, the delivery of plasmid DNA to express these fusion proteins via conventional transient… read more here.
Keywords: transfection; delivery; expression; fusion ... See more keywords
Advances in mRNA non-viral delivery approaches.
Sign Up to like & getrecommendations! Published in 2021 at "Advanced drug delivery reviews"
DOI: 10.1016/j.addr.2021.113930
Abstract: Messenger RNAs (mRNAs) present a great potential as therapeutics for the treatment and prevention of a wide range of human pathologies, allowing for protein replacement, vaccination, cancer immunotherapy, and genomic engineering. Despite advances in the… read more here.
Keywords: non viral; delivery approaches; advances mrna; delivery ... See more keywords
Non-viral delivery systems for CRISPR/Cas9-based genome editing: Challenges and opportunities.
Sign Up to like & getrecommendations! Published in 2018 at "Biomaterials"
DOI: 10.1016/j.biomaterials.2018.04.031
Abstract: In recent years, CRISPR (clustered regularly interspaced short palindromic repeat)/Cas (CRISPR-associated) genome editing systems have become one of the most robust platforms in basic biomedical research and therapeutic applications. To date, efficient in vivo delivery of… read more here.
Keywords: genome editing; non viral; crispr cas9; delivery ... See more keywords
Intracellular Communication between Synthetic Macromolecules.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.2c02793
Abstract: Non-viral delivery is an important strategy for selective and efficient gene therapy, immunization, and RNA interference, which overcomes problems of genotoxicity and inherent immunogenicity associated with viral vectors. Liposomes and polymers are compelling candidates as… read more here.
Keywords: non viral; viral delivery; communication; communication synthetic ... See more keywords
Approaches towards biomaterial-mediated gene editing for cancer immunotherapy.
Sign Up to like & getrecommendations! Published in 2022 at "Biomaterials science"
DOI: 10.1039/d2bm00806h
Abstract: Gene therapies are transforming treatment modalities for many human diseases and disorders, including those in ophthalmology, oncology, and nephrology. To maximize the clinical efficacy and safety of these treatments, consideration of both delivery materials and… read more here.
Keywords: viral delivery; gene editing; immune cells; gene ... See more keywords
Non-Viral Delivery of CRISPR/Cas Cargo to the Retina Using Nanoparticles: Current Possibilities, Challenges, and Limitations
Sign Up to like & getrecommendations! Published in 2022 at "Pharmaceutics"
DOI: 10.3390/pharmaceutics14091842
Abstract: The discovery of the CRISPR/Cas system and its development into a powerful genome engineering tool have revolutionized the field of molecular biology and generated excitement for its potential to treat a wide range of human… read more here.
Keywords: non viral; viral delivery; cargo retina; crispr cas ... See more keywords