Non‐PCR Ultrasensitive Detection of Viral RNA by a Nanoprobe‐Coupling Strategy: SARS‐CoV‐2 as an Example
Sign Up to like & getrecommendations! Published in 2022 at "Advanced Healthcare Materials"
DOI: 10.1002/adhm.202200031
Abstract: Developing efficient and highly sensitive diagnostic techniques for early detections of pathogenic viruses such as Severe Acute Respiratory Syndrome Coronavirus 2 (SARS‐CoV‐2) is vitally important for preventing its widespread. However, the conventional polymerase chain reaction… read more here.
Keywords: viral rna; detection; strategy; non pcr ... See more keywords
Sequestering the 5′‐cap for viral RNA packaging
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DOI: 10.1002/bies.202200104
Abstract: Many viruses evolved mechanisms for capping the 5′‐ends of their plus‐strand RNAs as a means of hijacking the eukaryotic messenger RNA (mRNA) splicing/translation machinery. Although capping is critical for replication, the RNAs of these viruses… read more here.
Keywords: sequestering cap; viral rna; rna; packaging ... See more keywords
Detectable SARS‐CoV‐2 viral RNA in feces of three children during recovery period of COVID‐19 pneumonia
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Medical Virology"
DOI: 10.1002/jmv.25795
Abstract: Coronavirus Disease 2019 (COVID‐19) is a newly emerging infectious disease caused by a novel coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2). After its first occurrence in Wuhan of China from December 2019, COVID‐19 rapidly… read more here.
Keywords: detectable sars; covid; cov viral; three cases ... See more keywords
The Transactions of NS3 and NS5 in Flaviviral RNA Replication.
Sign Up to like & getrecommendations! Published in 2018 at "Advances in experimental medicine and biology"
DOI: 10.1007/978-981-10-8727-1_11
Abstract: Dengue virus (DENV) replication occurs in virus-induced vesicles that contain the replication complex (RC) where viral RNA, viral proteins and host proteins participate in RNA-RNA, RNA-protein and protein-protein interactions to ensure viral genome synthesis. However,… read more here.
Keywords: protein; rna replication; rna; ns3 ns5 ... See more keywords
Treatment of hepatitis C virus infection with direct-acting antivirals plus ribavirin eliminates viral RNA from peripheral blood mononuclear cells and reduces virologic relapse in diverse hepatic parenchymal changes.
Sign Up to like & getrecommendations! Published in 2021 at "Archives of virology"
DOI: 10.1007/s00705-021-04969-4
Abstract: Elimination of hepatitis C virus (HCV) may fail, leading to a non-response outcome because of inappropriate testing for viral RNA in peripheral blood mononuclear cells (PBMCs). Sequelae of HCV genotype 4 therapy with sofosbuvir and… read more here.
Keywords: pbmcs; treatment; hcv; relapse ... See more keywords
Nasopharyngeal SARS-CoV-2 viral RNA shedding in patients with diabetes mellitus
Sign Up to like & getrecommendations! Published in 2020 at "Endocrine"
DOI: 10.1007/s12020-020-02516-w
Abstract: Dear Editor The paper by Buetti et al. [1] regarding the prolonged SARS-CoV-2 viral shedding in lower respiratory tract samples of critically ill patients presented a fascinating finding. In this Swiss cohort, the presence of… read more here.
Keywords: rna shedding; rna; diabetes mellitus; cov viral ... See more keywords
Cytologic and molecular correlates of SARS-CoV-2 infection of the nasopharynx
Sign Up to like & getrecommendations! Published in 2020 at "Annals of Diagnostic Pathology"
DOI: 10.1016/j.anndiagpath.2020.151565
Abstract: Abstract Infection by SARS-CoV-2 commonly begins in the nasopharynx, and the cytologic and molecular correlates are not characterized. Fifty-eight cytologic preps (20 oral and 38 from the nasopharynx) were obtained from ten patients and analyzed… read more here.
Keywords: cytologic molecular; molecular correlates; nasopharynx cytologic; infection ... See more keywords
Optimisation and evaluation of an automated system for extraction of viral RNA from oysters.
Sign Up to like & getrecommendations! Published in 2019 at "International journal of food microbiology"
DOI: 10.1016/j.ijfoodmicro.2019.108386
Abstract: The NucliSENS MiniMAG (Minimag) system from bioMérieux is widely used for extraction of viral RNA from oysters and is included as informative material in the ISO method for quantification of hepatitis A virus (HAV) and… read more here.
Keywords: system; norovirus; rna oysters; automated system ... See more keywords
Associations of clinical characteristics and treatment regimens with the duration of viral RNA shedding in patients with COVID-19
Sign Up to like & getrecommendations! Published in 2020 at "International Journal of Infectious Diseases"
DOI: 10.1016/j.ijid.2020.06.091
Abstract: Abstract Objective The novel coronavirus disease 2019 (COVID-19) has become a global pandemic, but the factors influencing viral RNA shedding remain unclear to inform optimal control strategies. Methods The clinical course and viral RNA shedding… read more here.
Keywords: within days; rna shedding; treatment; rna ... See more keywords
A simple and rapid approach to prepare Sindbis and West Nile viral RNA controls for differentiation between positive samples and laboratory contamination.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of virological methods"
DOI: 10.1016/j.jviromet.2020.113822
Abstract: Reverse transcription-polymerase chain reaction (RT-PCR) is frequently used for surveillance and diagnosis of arboviruses and emerging viruses. A disadvantage of RT-PCR assays, especially nested assays, is the potential for false-positive results caused by laboratory contamination… read more here.
Keywords: positive control; positive samples; contamination; laboratory contamination ... See more keywords
The tale of two flaviviruses: subversion of host pathways by RNA shapes in dengue and hepatitis C viral RNA genomes.
Sign Up to like & getrecommendations! Published in 2020 at "Current opinion in microbiology"
DOI: 10.1016/j.mib.2020.08.007
Abstract: Pathogenic RNA viruses continue to emerge owing to their rapid evolutionary rates. The family of the Flaviviridae contains enveloped, single-stranded, positive-sense RNA viruses that include mosquito borne viruses such as dengue virus and the blood-borne… read more here.
Keywords: tale two; two flaviviruses; rna; host ... See more keywords