Viral Vectors, Engineered Cells and the CRISPR Revolution.
Sign Up to like & getrecommendations! Published in 2017 at "Advances in experimental medicine and biology"
DOI: 10.1007/978-3-319-63904-8_1
Abstract: Over the past few decades the ability to edit human cells has revolutionized modern biology and medicine. With advances in genome editing methodologies, gene delivery and cell-based therapeutics targeted at treatment of genetic disease have… read more here.
Keywords: medicine; viral vectors; cells crispr; biology ... See more keywords
Viral vectors for neuronal cell type-specific visualization and manipulations
Sign Up to like & getrecommendations! Published in 2020 at "Current Opinion in Neurobiology"
DOI: 10.1016/j.conb.2020.03.011
Abstract: Characterizing neuronal cell types demands efficient strategies for specific labeling and manipulation of individual subtypes to dissect their connectivity and functions. Recombinant viral technology offers a powerful toolbox for targeted transgene expression in specific neuronal… read more here.
Keywords: viral vectors; type specific; neuronal cell; cell ... See more keywords
Methods for mechanical delivery of viral vectors into rhesus monkey brain
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Neuroscience Methods"
DOI: 10.1016/j.jneumeth.2020.108730
Abstract: BACKGROUND Modern molecular tools make it possible to manipulate neural activity in a reversible and cell-type specific manner. For rhesus monkey research, molecular tools are generally introduced via viral vectors. New instruments designed specifically for… read more here.
Keywords: rhesus monkey; vector; viral vectors; delivery ... See more keywords
New viral vectors for infectious diseases and cancer.
Sign Up to like & getrecommendations! Published in 2020 at "Seminars in immunology"
DOI: 10.1016/j.smim.2020.101430
Abstract: Since the discovery in 1796 by Edward Jenner of vaccinia virus as a way to prevent and finally eradicate smallpox, the concept of using a virus to fight another virus has evolved into the current… read more here.
Keywords: viral vectors; infectious diseases; diseases cancer; viral vectored ... See more keywords
Novel RNA Viral Vectors for Chemically Regulated Gene Expression in Embryonic Stem Cells.
Sign Up to like & getrecommendations! Published in 2021 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.1c00214
Abstract: RNA viral vectors that replicate without DNA intermediates are attractive platforms for manipulation of cells for biomedical and veterinary applications because they have minimal risk of chromosomal integration. Vesicular stomatitis virus (VSV) vectors are among… read more here.
Keywords: viral vectors; gene expression; rna viral; embryonic stem ... See more keywords
Tripartite polyionic complex (PIC) micelles as non-viral vectors for mesenchymal stem cell siRNA transfection.
Sign Up to like & getrecommendations! Published in 2017 at "Biomaterials science"
DOI: 10.1039/c7bm00384f
Abstract: In the context of regenerative medicine, the use of RNA interference mechanisms has already proven its efficiency in targeting specific gene expression with the aim of enhancing, accelerating or, more generally, directing stem cell differentiation.… read more here.
Keywords: viral vectors; transfection; tripartite polyionic; stem cell ... See more keywords
Pre-clinical non-viral vectors exploited for in vivo CRISPR/Cas9 gene editing: an overview.
Sign Up to like & getrecommendations! Published in 2022 at "Biomaterials science"
DOI: 10.1039/d1bm01452h
Abstract: Clustered regulatory interspaced short palindromic repeats or CRISPR/Cas9 has emerged as a potent and versatile tool for efficient genome editing. This technology has been exploited for several applications including disease modelling, cell therapy, diagnosis, and… read more here.
Keywords: gene editing; crispr cas9; viral vectors; crispr ... See more keywords
Overcoming barriers in non-viral gene delivery for neurological applications.
Sign Up to like & getrecommendations! Published in 2022 at "Nanoscale"
DOI: 10.1039/d1nr06939j
Abstract: Gene therapy for neurological disorders has attracted significant interest as a way to reverse or stop various disease pathologies. Typical gene therapies involving the central and peripheral nervous system make use of adeno-associated viral vectors… read more here.
Keywords: overcoming barriers; viral vectors; delivery; barriers non ... See more keywords
Dendron-functionalised hyperbranched bis-MPA polyesters as efficient non-viral vectors for gene therapy in different cell lines.
Sign Up to like & getrecommendations! Published in 2022 at "Biomaterials science"
DOI: 10.1039/d2bm00365a
Abstract: Gene therapy has become a relevant tool in the biomedical field to treat or even prevent some diseases. The effective delivery of genetic material into the cell remains a crucial step to succeed in this… read more here.
Keywords: non viral; cell; gene therapy; bis mpa ... See more keywords
Lipoplexes and polyplexes as nucleic acids delivery nanosystems: The current state and future considerations
Sign Up to like & getrecommendations! Published in 2022 at "Expert Opinion on Drug Delivery"
DOI: 10.1080/17425247.2022.2075846
Abstract: ABSTRACT Introduction Designing safe and effective nucleic acid delivery nanosystems presents a challenge that requires a good understanding of various biological barriers, whose impact is frequently neglected during in vitro assessments. Hence, the development of… read more here.
Keywords: non viral; viral vectors; lipoplexes polyplexes; polyplexes nucleic ... See more keywords
Molecular barcoding of viral vectors enables mapping and optimization of mRNA trans-splicing.
Sign Up to like & getrecommendations! Published in 2018 at "RNA"
DOI: 10.1261/rna.063925.117
Abstract: Genome editing has proven to be highly potent in the generation of functional gene knockouts in dividing cells. In the CNS however, efficient technologies to repair sequences are yet to materialize. Reprogramming on the mRNA… read more here.
Keywords: viral vectors; vectors enables; enables mapping; barcoding viral ... See more keywords