Modification and application of an in vitro assay to examine inositol phosphate degradation in the digestive tract of poultry.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of the science of food and agriculture"
DOI: 10.1002/jsfa.8297
Abstract: BACKGROUND An in vitro assay was modified to study the disappearance of inositol hexakisphosphate (InsP6 ) and the formation of lower inositol phosphate (InsP) isomers in the poultry digestive tract, and three experiments investigated the… read more here.
Keywords: inositol; vitro assay; inositol phosphate; digestive tract ... See more keywords
In Vitro Assay to Study Histone Ubiquitination During Transcriptional Regulation.
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DOI: 10.1007/978-1-4939-6518-2_17
Abstract: In mammals, gene expression is largely controlled at the transcriptional level. In response to environmental or intrinsic signaling, gene expression is often fine-tuned by epigenetic modifications, including DNA methylation and histone modifications. One such histone… read more here.
Keywords: histone ubiquitination; vitro assay; assay study; histone ... See more keywords
An Assay for Measuring Histone Variant Exchange within Nucleosomes In Vitro.
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DOI: 10.1007/978-1-4939-6630-1_2
Abstract: The incorporation of histone variants into specific chromatin regions is a mechanism by which cells can regulate many important biological processes. One such example is H2A.Z, a highly conserved variant of H2A that is incorporated… read more here.
Keywords: vitro assay; variant exchange; h2a; exchange ... See more keywords
In Vitro Assay for Induction of Adventitious Rooting on Intact Arabidopsis Hypocotyls.
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DOI: 10.1007/978-1-4939-7747-5_7
Abstract: Adventitious roots (AR) are de novo formed roots that emerge from any part of the plant or from callus in tissue culture, except root tissue. The plant tissue origin and the method by which they… read more here.
Keywords: assay induction; vitro assay; adventitious rooting; tissue ... See more keywords
Examining P‐gp efflux kinetics guided by the BDDCS ‐ Rational selection of in vitro assay designs and mathematical models
Sign Up to like & getrecommendations! Published in 2019 at "European Journal of Pharmaceutical Sciences"
DOI: 10.1016/j.ejps.2019.03.005
Abstract: ABSTRACT The generation of reliable kinetic parameters to describe P‐glycoprotein (P‐gp) activity is essential for predicting the impact of efflux transport on gastrointestinal drug absorption. The compound‐specific selection of in vitro assay designs and ensuing… read more here.
Keywords: assay designs; vitro assay; selection vitro; selection ... See more keywords
A cell-based in vitro assay for testing of immunological integrity of Tetanus toxoid vaccine antigen
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DOI: 10.1038/s41541-021-00344-1
Abstract: Vaccines containing inactivated toxins confer protection by eliciting a neutralizing antibody response against bacterial toxins such as tetanus and diphtheria. At present, release of tetanus toxoid (TT) and diphtheria toxoid (DT)-containing vaccines relies on in… read more here.
Keywords: vitro assay; tetanus toxoid; vaccine antigen; vaccine ... See more keywords
Synthesis and in vitro assay of hydroxyxanthones as antioxidant and anticancer agents
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DOI: 10.1038/s41598-022-05573-5
Abstract: In the present work, three hydroxyxanthones were synthesized in 11.15–33.42% yield from 2,6-dihydroxybenzoic acid as the starting material. The chemical structures of prepared hydroxyxanthones have been elucidated by using spectroscopic techniques. Afterward, the hydroxyxanthones were… read more here.
Keywords: antioxidant anticancer; anticancer agents; synthesis vitro; vitro assay ... See more keywords
A fast, miniaturised in-vitro assay developed for quantification of lipase enzyme activity
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Enzyme Inhibition and Medicinal Chemistry"
DOI: 10.1080/14756366.2019.1651312
Abstract: Abstract The discovery of allosteric modulators is a multi-disciplinary approach, which is time- and cost-intensive. High-throughput screening combined with novel computational tools can reduce these factors. Thus, we developed an enzyme activity assay, which can… read more here.
Keywords: vitro assay; lipase; fast miniaturised; enzyme activity ... See more keywords
Abstract 5338: A novel in vitro assay to evaluate the roles of hepatic metabolism on anticancer drug safety and efficacy
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DOI: 10.1158/1538-7445.am2023-5338
Abstract: It has been well-established that hepatic drug metabolism may have significant impact on the efficacy and off-target toxicity of anticancer drugs. We present here an in vitro assay, the metabolism-dependent cytotoxicity assay (MDCA), for the… read more here.
Keywords: metabolism; human hepatocytes; vitro assay; novel vitro ... See more keywords
In Vitro Assay Development and HTS of Small-Molecule Human ABAD/17β-HSD10 Inhibitors as Therapeutics in Alzheimer’s Disease
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DOI: 10.1177/2472555217697964
Abstract: A major hallmark of Alzheimer’s disease (AD) is the formation of neurotoxic aggregates composed of the amyloid-β peptide (Aβ). Aβ has been recognized to interact with numerous proteins, resulting in pathological changes to the metabolism… read more here.
Keywords: abad; alzheimer disease; vitro assay; assay development ... See more keywords
Role of miRNA-99a-5p in modulating the functionof hepatocellular carcinoma cells: Bioinformatics Analysis and In Vitro assay.
Sign Up to like & getrecommendations! Published in 2023 at "Current cancer drug targets"
DOI: 10.2174/1568009623666230202155440
Abstract: AIM This study aimed to investigate the biological functions of miRNAs in hepatobiliary tumors as the focus of targeted therapy research. BACKGROUND Hepatobiliary tumors are among the leading causes of cancer-related deaths worldwide. Many microRNAs… read more here.
Keywords: cancer; vitro assay; role; analysis ... See more keywords