In Vitro Assembly of Nucleosomes for Binding/Remodeling Assays.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6630-1_1
Abstract: In order to study the functions and activities of chromatin remodeling enzymes in vitro, it is necessary to be able to reconstitute nucleosomes on DNA templates. In this chapter, we describe procedures for purification of… read more here.
Keywords: nucleosomes binding; vitro assembly; remodeling assays; biology ... See more keywords
In vitro assembly of chimeric virus-like particles composed of a porcine circovirus 2b capsid protein and a B-cell epitope of infectious bursal disease virus.
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology letters"
DOI: 10.1007/s10529-022-03237-y
Abstract: OBJECTIVES To develop a method for in vitro assembly of recombinant proteins expressed in E. coli into chimeric virus-like particles (cVLPs). RESULTS A fusion protein (Bepi-Cap-A) between capsid protein (Cap) of PCV2b and B cell… read more here.
Keywords: cap; virus like; vitro assembly; like particles ... See more keywords
An In Vitro Assembly System Identifies Roles for RNA Nucleation and ATP in Yeast Stress Granule Formation.
Sign Up to like & getrecommendations! Published in 2020 at "Molecular cell"
DOI: 10.1016/j.molcel.2020.07.017
Abstract: Stress granules (SGs) are condensates of mRNPs that form in response to stress. SGs arise by multivalent protein-protein, protein-RNA, and RNA-RNA interactions. However, the role of RNA-RNA interactions in SG assembly remains understudied. Here, we… read more here.
Keywords: assembly system; formation; vitro assembly; stress ... See more keywords
In Vitro Assembly of Diverse Bacterial Microcompartment Shell Architectures.
Sign Up to like & getrecommendations! Published in 2018 at "Nano letters"
DOI: 10.1021/acs.nanolett.8b02991
Abstract: Bacterial microcompartments (BMCs) are organelles composed of a selectively permeable protein shell that encapsulates enzymes involved in CO2 fixation (carboxysomes) or carbon catabolism (metabolosomes). Confinement of sequential reactions by the BMC shell presumably increases the… read more here.
Keywords: diverse bacterial; vitro assembly; bacterial microcompartment; assembly diverse ... See more keywords
Near-physiological in vitro assembly of 50S ribosomes involves parallel pathways
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1101/2022.11.09.515862
Abstract: Understanding the assembly principles of biological macromolecular complexes remains a significant challenge, due to the complexity of the systems and the difficulties in developing experimental approaches. As a ribonucleoprotein complex, the ribosome serves as an… read more here.
Keywords: vitro assembly; assembly; near physiological; physiological vitro ... See more keywords
The interaction of dengue virus capsid protein with negatively charged interfaces drives the in vitro assembly of nucleocapsid-like particles
Sign Up to like & getrecommendations! Published in 2022 at "PLoS ONE"
DOI: 10.1371/journal.pone.0264643
Abstract: Dengue virus (DENV) causes a major arthropod-borne viral disease, with 2.5 billion people living in risk areas. DENV consists in a 50 nm-diameter enveloped particle in which the surface proteins are arranged with icosahedral symmetry,… read more here.
Keywords: capsid protein; nucleocapsid like; like particles; vitro assembly ... See more keywords
In vitro assembly of plasmid DNA for direct cloning in Lactiplantibacillus plantarum WCSF1
Sign Up to like & getrecommendations! Published in 2023 at "PLOS ONE"
DOI: 10.1371/journal.pone.0281625
Abstract: Lactobacilli are gram-positive bacteria that are growing in importance for the healthcare industry and genetically engineering them as living therapeutics is highly sought after. However, progress in this field is hindered since most strains are… read more here.
Keywords: plantarum; dna; vitro assembly; assembly plasmid ... See more keywords