cDNA Display of Disulfide-Containing Peptide Library and In Vitro Evolution.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9853-1_4
Abstract: Directed in vitro evolution (IVE) is now a widely applied technology to obtain molecules that have designed new features of one's demands. We describe here experimental procedures for a cDNA display IVE to select peptide… read more here.
Keywords: evolution; vitro evolution; peptide library; disulfide containing ... See more keywords
One-Shot In Vitro Evolution Generated an Antibody Fragment for Testing Urinary Cotinine with More Than 40-Fold Enhanced Affinity.
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DOI: 10.1021/acs.analchem.6b04332
Abstract: Immunoassays for cotinine, a major nicotine metabolite, in the urine are useful for monitoring the degree of tobacco smoke exposure. However, hybridoma-based anti-cotinine antibodies lack sufficient binding affinity to perform practically sensitive measurements, and thus… read more here.
Keywords: urinary cotinine; vitro evolution; one shot; cotinine ... See more keywords
PEI-assisted boronate affinity magnetic nanoparticle-based SELEX for efficient in vitro evolution of saponin-binding aptamers
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DOI: 10.1039/d1ra00889g
Abstract: Saponin is a large family of important natural products with various pharmacological activities. Selective enrichment of saponin from complex biological samples is a key step for analysis of saponin. Despite that aptamers have been widely… read more here.
Keywords: vitro evolution; evolution saponin; saponin; affinity magnetic ... See more keywords
In vitro evolution of ribonucleases from expanded genetic alphabets
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2208261119
Abstract: Significance The ability of nucleic acids to catalyze reactions is important in the context of the origin of life and biomedical applications. However, the catalytic power of standard nucleic acids assembled from just four nucleotide… read more here.
Keywords: expanded genetic; evolution ribonucleases; genetic alphabets; vitro evolution ... See more keywords
Structural transition of replicable RNAs during in vitro evolution with Qβ replicase.
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DOI: 10.1261/rna.073106.119
Abstract: Single-stranded RNAs (ssRNAs) are utilized as genomes in some viruses and also in experimental models of ancient life-forms owing to the simplicity. One of the largest problems for ssRNA replication is the formation of double-stranded… read more here.
Keywords: vitro evolution; formation; dsrna formation; ssrna replication ... See more keywords