Decreased CRISPLD2 expression impairs osteogenic differentiation of human mesenchymal stem cells during in vitro expansion.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of cellular physiology"
DOI: 10.1002/jcp.31014
Abstract: Human mesenchymal stem cells (hMSCs) are the cornerstone of regenerative medicine; large quantities of hMSCs are required via in vitro expansion to meet therapeutic purposes. However, hMSCs quickly lose their osteogenic differentiation potential during in… read more here.
Keywords: expansion; stem cells; osteogenic differentiation; vitro expansion ... See more keywords
Microfluidic-Printed Microcarrier for In Vitro Expansion of Adherent Stem Cells in 3D Culture Platform.
Sign Up to like & getrecommendations! Published in 2019 at "Macromolecular bioscience"
DOI: 10.1002/mabi.201900136
Abstract: Microcarrier-based stem cell expansion cultures can increase the dimensions of in vitro stem cell cultures from 2D to 3D. The culture handling process then becomes more efficient compared with conventional 2D cultures. However, the use… read more here.
Keywords: expansion; vitro expansion; platform; stem ... See more keywords
Extracellular matrix derived from chondrocytes promotes rapid expansion of human primary chondrocytes in vitro with reduced dedifferentiation.
Sign Up to like & getrecommendations! Published in 2019 at "Acta biomaterialia"
DOI: 10.1016/j.actbio.2018.12.006
Abstract: A significant expansion of autologous chondrocytes in vitro is required for cell-based cartilage repair. However, the in vitro expansion of chondrocytes under standard culture conditions inevitably leads to the dedifferentiation of chondrocytes and contributes to… read more here.
Keywords: expansion; microscopy; vitro expansion; chondrocytes vitro ... See more keywords
Platelet lysate reduces the chondrocyte dedifferentiation during in vitro expansion: Implications for cartilage tissue engineering.
Sign Up to like & getrecommendations! Published in 2020 at "Research in veterinary science"
DOI: 10.1016/j.rvsc.2020.08.017
Abstract: In vitro studies have demonstrated that platelet lysate (PL) can serve as an alternative to platelet-rich plasma (PRP) to sustain chondrocyte proliferation and production of extracellular matrix components in chondrocytes. The present study aimed to… read more here.
Keywords: vitro expansion; proliferation; platelet lysate; platelet ... See more keywords
Low Intensity Vibrations Augment Mesenchymal Stem Cell Proliferation and Differentiation Capacity during in vitro Expansion
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-66055-0
Abstract: A primary component of exercise, mechanical signals, when applied in the form of low intensity vibration (LIV), increases mesenchymal stem cell (MSC) osteogenesis and proliferation. While it is generally accepted that exercise effectively combats the… read more here.
Keywords: proliferation; stem cell; cell; vitro expansion ... See more keywords
Cellular expansion of MSCs: Shifting the regenerative potential
Sign Up to like & getrecommendations! Published in 2022 at "Aging Cell"
DOI: 10.1111/acel.13759
Abstract: Mesenchymal‐derived stromal or progenitor cells, commonly called “MSCs,” have attracted significant clinical interest for their remarkable abilities to promote tissue regeneration and reduce inflammation. Recent studies have shown that MSCs' therapeutic effects, originally attributed to… read more here.
Keywords: cellular expansion; expansion mscs; mscs shifting; mscs ... See more keywords
An Approach towards a GMP Compliant In-Vitro Expansion of Human Adipose Stem Cells for Autologous Therapies
Sign Up to like & getrecommendations! Published in 2020 at "Bioengineering"
DOI: 10.3390/bioengineering7030077
Abstract: Human Adipose Tissue Stem Cells (hASCs) are a valuable source of cells for clinical applications (e.g., treatment of acute myocardial infarction and inflammatory diseases), especially in the field of regenerative medicine. However, for autologous (patient-specific)… read more here.
Keywords: human adipose; cell; expansion; vitro expansion ... See more keywords
Effect of Human Platelet Lysate as Cultivation Nutrient Supplement on Human Natal Dental Pulp Stem Cell In Vitro Expansion
Sign Up to like & getrecommendations! Published in 2022 at "Biomolecules"
DOI: 10.3390/biom12081091
Abstract: Despite several scientific or ethical issues, fetal bovine serum (FBS) remains the standard nutrient supplement in the mesenchymal stem cell cultivation medium. Cell amplification plays an important role in human stem cell therapies. Increasing interest… read more here.
Keywords: cell; stem cell; supplement; stem ... See more keywords
High quality in vitro expansion of human endothelial progenitor cells of human umbilical vein origin
Sign Up to like & getrecommendations! Published in 2017 at "International Journal of Medical Sciences"
DOI: 10.7150/ijms.18137
Abstract: The limited availability of qualified endothelial progenitor cells (EPCs) is a major challenge for regenerative medicine. In the present study, we isolated human EPCs from human umbilical vein endothelial cells (HUVECs) by using magnetic micro-beads… read more here.
Keywords: medicine; progenitor cells; vitro expansion; human umbilical ... See more keywords