In vitro reconstitution of small GTPase regulation
Sign Up to like & getrecommendations! Published in 2022 at "FEBS Letters"
DOI: 10.1002/1873-3468.14540
Abstract: Small GTPases play essential roles in the organization of eukaryotic cells. In recent years, it has become clear that their intracellular functions result from intricate biochemical networks of the GTPase and their regulators that dynamically… read more here.
Keywords: vitro reconstitution; small gtpase; gtpase; reconstitution small ... See more keywords
Visualizing Autophagic Lysosome Reformation in Cells Using In Vitro Reconstitution Systems
Sign Up to like & getrecommendations! Published in 2018 at "Current Protocols in Cell Biology"
DOI: 10.1002/cpcb.44
Abstract: Autophagy is a lysosome‐based degradation pathway. Autophagic lysosome reformation (ALR) is a lysosomal membrane recycling process that marks the terminal step of autophagy. During ALR, LAMP1‐positive tubules, named reformation tubules, are extruded from autolysosomes, and… read more here.
Keywords: reformation; vitro reconstitution; lysosome reformation; autophagic lysosome ... See more keywords
Studying Mitochondrial Network Formation by In Vivo and In Vitro Reconstitution Assay.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1266-8_25
Abstract: Mitochondria change their morphologies from small isolated vesicles to large continuous networks across the cell cycles. The mitochondrial network formation (MNF) plays an important role in maintaining mitochondrial DNA integrity and interchanging mitochondrial materials. The… read more here.
Keywords: network formation; network; vivo; mitochondrial network ... See more keywords
In vitro reconstitution of translational arrest pathways.
Sign Up to like & getrecommendations! Published in 2018 at "Methods"
DOI: 10.1016/j.ymeth.2017.12.018
Abstract: Protein translation is tightly regulated to ensure high-fidelity expression of genetic information. Various conditions cause ribosomes to stall while synthesizing new proteins. Different types of translational arrest initiate specific mRNA surveillance, protein quality control, and… read more here.
Keywords: vitro reconstitution; arrest pathways; translational arrest; arrest ... See more keywords
In Vitro Reconstitution of Fimsbactin Biosynthesis from Acinetobacter baumannii.
Sign Up to like & getrecommendations! Published in 2022 at "ACS chemical biology"
DOI: 10.1021/acschembio.2c00573
Abstract: Siderophores produced via nonribosomal peptide synthetase (NRPS) pathways serve as critical virulence factors for many pathogenic bacteria. Improved knowledge of siderophore biosynthesis guides the development of inhibitors, vaccines, and other therapeutic strategies. Fimsbactin A is… read more here.
Keywords: biosynthesis; vitro reconstitution; reconstitution fimsbactin; acinetobacter baumannii ... See more keywords
In vivo and in vitro reconstitution of unique key steps in cystobactamid antibiotic biosynthesis
Sign Up to like & getrecommendations! Published in 2021 at "Nature Communications"
DOI: 10.1038/s41467-021-21848-3
Abstract: Cystobactamids are myxobacteria-derived topoisomerase inhibitors with potent anti-Gram-negative activity. They are formed by a non-ribosomal peptide synthetase (NRPS) and consist of tailored para-aminobenzoic acids, connected by a unique α-methoxy-l-isoasparagine or a β-methoxy-l-asparagine linker moiety. We… read more here.
Keywords: vivo vitro; biosynthesis; vitro reconstitution; reconstitution unique ... See more keywords
In vitro reconstitution and characterisation of the oxidative d-xylose pathway for production of organic acids and alcohols
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DOI: 10.1186/s13568-019-0768-7
Abstract: The oxidative d-xylose pathway, i.e. Dahms pathway, can be utilised to produce from cheap biomass raw material useful chemical intermediates. In vitro metabolic pathways offer a fast way to study the rate-limiting steps and find… read more here.
Keywords: vitro reconstitution; reconstitution characterisation; xylose pathway; pathway ... See more keywords
In Vitro Reconstitution of the Melanin Pathway’s Catalytic Activities Using Tyrosinase Nanoparticles
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms24010639
Abstract: The melanogenesis pathway is characterized by a series of reactions catalyzed by key enzymes, such as tyrosinase (TYR), tyrosinase-related protein 2 (TYRP2), and tyrosinase-related protein 1 (TYRP1), to produce melanin pigment. However, in vitro studies… read more here.
Keywords: vitro reconstitution; melanin; reconstitution melanin; tyrosinase ... See more keywords
Author response: In vitro reconstitution reveals major differences between human and bacterial cytochrome c synthases
Sign Up to like & getrecommendations! Published in 2021 at "eLife"
DOI: 10.7554/elife.64891.sa2
Abstract: Cytochromes c are ubiquitous heme proteins in mitochondria and bacteria, all possessing a CXXCH (CysXxxXxxCysHis) motif with covalently attached heme. We describe the first in vitro reconstitution of cytochrome c biogenesis using purified mitochondrial (HCCS)… read more here.
Keywords: cytochrome synthases; peptide analogs; vitro reconstitution; cytochrome ... See more keywords