In Vitro-Transcribed (IVT)-mRNA CAR Therapy Development.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0146-4_7
Abstract: Chimeric antigen receptor (CAR) cancer immunotherapy uses autologous immune system's cells, genetically modified, to reinforce the immune system against cancer cells. Genetic modification is usually mediated via viral transfection, despite the risk of insertional oncogenesis… read more here.
Keywords: transcribed ivt; ivt mrna; car; car therapy ... See more keywords
Exploring the Impact of In Vitro-Transcribed mRNA Impurities on Cellular Responses
Sign Up to like & getrecommendations! Published in 2024 at "Analytical Chemistry"
DOI: 10.1021/acs.analchem.4c04162
Abstract: Advances in mRNA technology have enabled mRNA-based therapies to enter a new era of medicine. Such therapies benefit from a single, standardized in vitro transcription (IVT) manufacturing process applicable to a wide range of targets.… read more here.
Keywords: impact vitro; cellular responses; exploring impact; vitro transcribed ... See more keywords
In vitro-transcribed antigen receptor mRNA nanocarriers for transient expression in circulating T cells in vivo
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-19486-2
Abstract: Engineering chimeric antigen receptors (CAR) or T cell receptors (TCR) helps create disease-specific T cells for targeted therapy, but the cost and rigor associated with manufacturing engineered T cells ex vivo can be prohibitive, so… read more here.
Keywords: cells vivo; circulating cells; transcribed antigen; vitro transcribed ... See more keywords
Optimizing the use of in vitro transcribed SGK1-mRNA as a therapeutic tool to treat female infertility
Sign Up to like & getrecommendations! Published in 2025 at "BMC Research Notes"
DOI: 10.1186/s13104-025-07346-5
Abstract: Gene therapy has emerged as an encouraging therapeutic approach for several diseases. The use of plasmid DNA (pDNA) or viral vectors encoding proteins have yielded low efficacy in recent trials. Synthetic messenger RNA (mRNA), also… read more here.
Keywords: ivt mrna; optimizing use; sgk1; vitro transcribed ... See more keywords
Translation of in vitro-transcribed RNA therapeutics
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Molecular Biosciences"
DOI: 10.3389/fmolb.2023.1128067
Abstract: In vitro transcribed, modified messenger RNAs (IVTmRNAs) have been used to vaccinate billions of individuals against the SARS-CoV-2 virus, and are currently being developed for many additional therapeutic applications. IVTmRNAs must be translated into proteins… read more here.
Keywords: vitro; vitro transcribed; rna therapeutics; translation vitro ... See more keywords