Increasing yield of in vitro transcription reaction with at‐line high pressure liquid chromatography monitoring
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28299
Abstract: The COVID‐19 pandemic triggered an unprecedented rate of development of messenger ribonucleic acid (mRNA) vaccines, which are produced by in vitro transcription reactions. The latter has been the focus of intense development to increase productivity… read more here.
Keywords: reaction; line high; monitoring; batch ... See more keywords
Application of the SSB biosensor to study in vitro transcription
Sign Up to like & getrecommendations! Published in 2018 at "Biochemical and Biophysical Research Communications"
DOI: 10.1016/j.bbrc.2018.01.147
Abstract: Gene expression, catalysed by RNA polymerases (RNAP), is one of the most fundamental processes in living cells. The majority of methods to quantify mRNA are based upon purification of the nucleic acid which leads to… read more here.
Keywords: biosensor; ssb biosensor; vitro transcription; study ... See more keywords
Real-Time Visualization of in Vitro Transcription of a Fluorescent RNA Aptamer: An Experiment for the Upper-Division Undergraduate or First-Year Graduate Laboratory
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Chemical Education"
DOI: 10.1021/acs.jchemed.7b00735
Abstract: A teaching laboratory experiment is described where students prepare in vitro transcription reactions of a fluorescent RNA aptamer, named Broccoli, and observe the production of the aptamer in real... read more here.
Keywords: fluorescent rna; vitro transcription; experiment; real time ... See more keywords
Vitamin-guanosine monophosphate conjugates for in vitro transcription priming.
Sign Up to like & getrecommendations! Published in 2020 at "Chemical communications"
DOI: 10.1039/c9cc09427j
Abstract: Expanding the catalytic repertoire of ribozymes to include vitamin synthesis requires efficient labelling of RNA with the substrate of interest, prior to in vitro selection. For this purpose, we rationally designed and synthesized six GMP-conjugates… read more here.
Keywords: guanosine monophosphate; transcription; vitro transcription; conjugates vitro ... See more keywords
Psychrophilic phage VSW-3 RNA polymerase reduces both terminal and full-length dsRNA byproducts in in vitro transcription
Sign Up to like & getrecommendations! Published in 2022 at "RNA Biology"
DOI: 10.1080/15476286.2022.2139113
Abstract: ABSTRACT RNA research and applications are underpinned by in vitro transcription (IVT), but RNA impurities resulting from the enzymatic reagents severely impede downstream applications. To improve the stability and purity of synthesized RNA, we have… read more here.
Keywords: vsw; transcription; rnap; rna polymerase ... See more keywords
In vitro transcription–translation using bacterial genome as a template to reconstitute intracellular profile
Sign Up to like & getrecommendations! Published in 2017 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkx776
Abstract: Abstract In vitro transcription–translation systems (TX–TL) can synthesize most of individual genes encoded in genomes by using strong promoters and translation initiation sequences. This fact raises a possibility that TX–TL using genome as a template… read more here.
Keywords: transcription; transcription translation; vitro transcription; genome template ... See more keywords
Synthesis of Single-Stranded RNA Probes by In Vitro Transcription.
Sign Up to like & getrecommendations! Published in 2020 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot100628
Abstract: In this protocol, we describe procedures for synthesizing RNA probes of high specific activity from DNA templates containing promoters for bacteriophage-encoded RNA polymerases. The DNA fragment to be transcribed should have previously been cloned into… read more here.
Keywords: single stranded; vitro transcription; synthesis single; rna probes ... See more keywords