Volta phase plate data collection facilitates image processing and cryo-EM structure determination.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of structural biology"
DOI: 10.1016/j.jsb.2018.01.003
Abstract: A current bottleneck in structure determination of macromolecular complexes by cryo electron microscopy (cryo-EM) is the large amount of data needed to obtain high-resolution 3D reconstructions, including through sorting into different conformations and compositions with… read more here.
Keywords: structure; biology; volta phase; image processing ... See more keywords
Single Particle Imaging with the Volta Phase Plate
Sign Up to like & getrecommendations! Published in 2019 at "Microscopy and Microanalysis"
DOI: 10.1017/s1431927619000011
Abstract: The Volta phase plate (VPP) is a remarkably simple device comprising a thin (~10 nm) amorphous carbon film positioned at the back focal plane of the objective lens (Fig. 1) [1]. The film is continuously… read more here.
Keywords: volta phase; phase shift; phase plate; film ... See more keywords