In-Chamber Reel-to-Reel System for Random-Access Volume Electron Microscopy
Sign Up to like & getrecommendations! Published in 2017 at "Microscopy and Microanalysis"
DOI: 10.1017/s1431927617001453
Abstract: Brain-wide synaptic wiring diagrams will be necessary to accurately predict brain-wide circuit computations underlying the diverse behaviors produced by an organism. Currently only volume electron microscopy (VEM) provides the nanometer-scale resolution required to reliably and… read more here.
Keywords: chamber reel; reel reel; volume; microscopy ... See more keywords
Automated synaptic connectivity inference for volume electron microscopy
Sign Up to like & getrecommendations! Published in 2017 at "Nature Methods"
DOI: 10.1038/nmeth.4206
Abstract: Teravoxel volume electron microscopy data sets from neural tissue can now be acquired in weeks, but data analysis requires years of manual labor. We developed the SyConn framework, which uses deep convolutional neural networks and… read more here.
Keywords: synaptic connectivity; electron microscopy; volume electron; microscopy ... See more keywords
How innovations in methodology offer new prospects for volume electron microscopy
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Microscopy"
DOI: 10.1111/jmi.13134
Abstract: Detailed knowledge of biological structure has been key in understanding biology at several levels of organisation, from organs to cells and proteins. Volume electron microscopy (volume EM) provides high resolution 3D structural information about tissues… read more here.
Keywords: electron microscopy; methodology; microscopy; volume electron ... See more keywords
A versatile enhanced freeze-substitution protocol for volume electron microscopy
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Cell and Developmental Biology"
DOI: 10.3389/fcell.2022.933376
Abstract: Volume electron microscopy, a powerful approach to generate large three-dimensional cell and tissue volumes at electron microscopy resolutions, is rapidly becoming a routine tool for understanding fundamental and applied biological questions. One of the enabling… read more here.
Keywords: freeze substitution; volume electron; microscopy; electron microscopy ... See more keywords
Large Volume Electron Microscopy and Neural Microcircuit Analysis
Sign Up to like & getrecommendations! Published in 2018 at "Frontiers in Neural Circuits"
DOI: 10.3389/fncir.2018.00098
Abstract: One recent technical innovation in neuroscience is microcircuit analysis using three-dimensional reconstructions of neural elements with a large volume Electron microscopy (EM) data set. Large-scale data sets are acquired with newly-developed electron microscope systems such… read more here.
Keywords: microcircuit analysis; large volume; volume electron; microscopy ... See more keywords