Exploiting the Twisted Intramolecular Charge Transfer Effect to Construct a Wash-Free Solvatochromic Fluorescent Lipid Droplet Probe for Fatty Liver Disease Diagnosis.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.1c04847
Abstract: The prominent pathological feature of fatty liver disease lesions is excessive fat accumulation in lipid droplets in hepatocytes. Thus, developing fluorescent lipid droplet-specific probes with high permeability and a high imaging contrast provides a robust… read more here.
Keywords: liver disease; probe; wash free; fatty liver ... See more keywords
Rapid and Wash-Free Time-Gated FRET Histamine Assays Using Antibodies and Aptamers.
Sign Up to like & getrecommendations! Published in 2022 at "ACS sensors"
DOI: 10.1021/acssensors.2c00085
Abstract: Histamine (HA) is an indicator of food freshness and quality. However, high concentrations of HA can cause food poisoning. Simple, rapid, sensitive, and specific quantification can enable efficient screening of HA in food and beverages.… read more here.
Keywords: rapid wash; time; food; histamine ... See more keywords
Affimer-enzyme-inhibitor switch sensor for rapid wash-free assays of multimeric proteins.
Sign Up to like & getrecommendations! Published in 2019 at "ACS sensors"
DOI: 10.1021/acssensors.9b01574
Abstract: Robust technology is required to underpin rapid point-of-care and in-field diagnostics, to improve timely decision making across broad sectors. An attractive strategy combines target recognition and signal generating elements into an "active" enzyme-switch that directly… read more here.
Keywords: inhibitor; enzyme inhibitor; inhibitor switch; switch sensor ... See more keywords
Wash-free colorimetric homogeneous immunoassay for Zygosaccharomyces rouxii
Sign Up to like & getrecommendations! Published in 2017 at "RSC Advances"
DOI: 10.1039/c7ra02791e
Abstract: Convenient, rapid and reliable detection of food spoilage microorganisms has always been critical to food safety. Commonly used methods such as an enzyme-linked immunosorbent assay (ELISA) often rely on tedious wash steps and incubation procedures.… read more here.
Keywords: free colorimetric; zygosaccharomyces rouxii; colorimetric homogeneous; wash free ... See more keywords
Fluorescent turn-on probes for wash-free mRNA imaging via covalent site-specific enzymatic labeling††Electronic supplementary information (ESI) available. See DOI: 10.1039/c7sc03150e Click here for additional data file.
Sign Up to like & getrecommendations! Published in 2017 at "Chemical Science"
DOI: 10.1039/c7sc03150e
Abstract: Investigating the many roles RNA plays in cellular regulation and function has increased demand for tools to explore RNA tracking and localization within cells. read more here.
Keywords: mrna imaging; probes wash; free mrna; turn probes ... See more keywords
Recognition triggered assembly of split aptamers to initiate a hybridization chain reaction for wash-free and amplified detection of exosomes.
Sign Up to like & getrecommendations! Published in 2020 at "Chemical communications"
DOI: 10.1039/d0cc02337j
Abstract: Here, we develop a facile split aptamer-based system for the amplified, specific and wash-free detection of exosomes in situ assisted by a target-induced hybridization chain reaction (HCR). This design was successfully used to detect target… read more here.
Keywords: detection exosomes; hybridization chain; wash free; chain reaction ... See more keywords