Miniaturized weak affinity chromatography for ligand identification of nanodiscs-embedded G-protein coupled receptors.
Sign Up to like & getrecommendations! Published in 2020 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2020.03.062
Abstract: Biophysical techniques that enable the screening and identification of weak affinity fragments against a target protein are at the heart of Fragment Based Drug Design approaches. In the case of membrane proteins, the crucial criteria… read more here.
Keywords: protein; affinity; weak affinity; nanodiscs embedded ... See more keywords
Monolith weak affinity chromatography for &mgr;g‐protein‐ligand interaction study
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Pharmaceutical and Biomedical Analysis"
DOI: 10.1016/j.jpba.2019.01.012
Abstract: &NA; Affinity monolith columns of 375 nL (effective length 8.5 cm, internal diameter 75 &mgr;m) were developed for protein‐ligand affinity investigations needing only 3 &mgr;g of human serum albumin (HSA). To promote specific interactions and… read more here.
Keywords: protein; affinity; weak affinity; affinity chromatography ... See more keywords