Cryopreservation of primary cultures of mammalian somatic cells in 96-well plates benefits from control of ice nucleation
Sign Up to like & getrecommendations! Published in 2020 at "Cryobiology"
DOI: 10.1016/j.cryobiol.2020.02.008
Abstract: Cryopreservation of mammalian cells has to date typically been conducted in cryovials, but there are applications where cryopreservation of primary cells in multiwell plates would be advantageous. However excessive supercooling in the small volumes of… read more here.
Keywords: well plates; ice nucleation; cells well; nucleation ... See more keywords
Segmenting Microscopy Images of Multi-Well Plates Based on Image Contrast
Sign Up to like & getrecommendations! Published in 2017 at "Microscopy and Microanalysis"
DOI: 10.1017/s1431927617012375
Abstract: Abstract Image segmentation is a key process in analyzing biological images. However, it is difficult to detect the differences between foreground and background when the image is unevenly illuminated. The unambiguous segmenting of multi-well plate… read more here.
Keywords: well plates; image; multi well; microscopy ... See more keywords
Quantitation of Trastuzumab and an Antibody to SARS-CoV-2 in Minutes Using Affinity Membranes in 96-Well Plates
Sign Up to like & getrecommendations! Published in 2022 at "Analytical Chemistry"
DOI: 10.1021/acs.analchem.1c03654
Abstract: Quantitation of therapeutic monoclonal antibodies (mAbs) in human serum could ensure that patients have adequate levels of mAbs for effective treatment. This research describes the use of affinity, glass-fiber membranes in a 96-well-plate format for… read more here.
Keywords: mab; affinity; quantitation; well plates ... See more keywords
Tethered primary hepatocyte spheroids on polystyrene multi-well plates for high-throughput drug safety testing
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-61699-4
Abstract: Hepatocyte spheroids are useful models for mimicking liver phenotypes in vitro because of their three-dimensionality. However, the lack of a biomaterial platform which allows the facile manipulation of spheroid cultures on a large scale severely… read more here.
Keywords: well plates; throughput drug; hepatocyte spheroids; polystyrene ... See more keywords
High-throughput formation and image-based analysis of basal-in mammary organoids in 384-well plates
Sign Up to like & getrecommendations! Published in 2022 at "Scientific Reports"
DOI: 10.1038/s41598-021-03739-1
Abstract: This manuscript describes a new method for forming basal-in MCF10A organoids using commercial 384-well ultra-low attachment (ULA) microplates and the development of associated live-cell imaging and automated analysis protocols. The use of a commercial 384-well… read more here.
Keywords: analysis; image; well plates; basal ... See more keywords
Design and testing of a centrifugal fluidic device for populating microarrays of spheroid cancer cell cultures
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Biological Engineering"
DOI: 10.1186/s13036-020-0228-6
Abstract: Background In current cancer spheroid culturing methods, the transfer and histological processing of specimens grown in 96-well plates is a time consuming process. A centrifugal fluidic device was developed and tested for rapid extraction of… read more here.
Keywords: well plates; planarity; centrifugal fluidic; fluidic device ... See more keywords
A 96-Well Plates-based UV Spectrophotometric Assay for Determination of Total Glucosinolates in Isatis indigotica Roots
Sign Up to like & getrecommendations! Published in 2021 at "Current Analytical Chemistry"
DOI: 10.2174/1573411017666210809094602
Abstract: Glucosinolates (GLS) are important secondary metabolites in Cruciferae vegetables and herbs. Currently, the assays of total GLS determination are cumbersome (requiring acidic or enzymatic hydrolysis and addition of staining reagents), time-consuming, and indirect. High concentrations… read more here.
Keywords: determination total; well plates; indigotica roots; determination ... See more keywords
Tailoring the HHx monomer content of P(HB-co-HHx) by flexible substrate compositions: scale-up from deep-well-plates to laboratory bioreactor cultivations
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2023.1081072
Abstract: The enhanced material properties exhibited by the microbially synthetized polyhydroxyalkanoate (PHA) copolymer poly(hydroxybutyrate-co-hydroxyhexanoate) [P(HB-co-HHx)] evidence that this naturally biodegrading biopolymer could replace various functionalities of established petrochemical plastics. In fact, the thermal processability, toughness and… read more here.
Keywords: scale deep; well plates; bioreactor cultivations; hhx ... See more keywords