Spreader‐CDR system for efficient, reproducible, and frugal western blot
Sign Up to like & getrecommendations! Published in 2021 at "ELECTROPHORESIS"
DOI: 10.1002/elps.202000380
Abstract: Efficient antibody incubation is a vital step for successful western blot. During the incubation, a thin antibody‐depleted layer is created around the blotting membrane, which limits antibody binding. Although the conventional batch shaking method is… read more here.
Keywords: spreader; incubation; antibody; western blot ... See more keywords
Western blot normalization: Time to choose a proper loading control seriously
Sign Up to like & getrecommendations! Published in 2023 at "ELECTROPHORESIS"
DOI: 10.1002/elps.202200222
Abstract: Recent research has questioned the validity of housekeeping proteins in Western blot. Our present study proposed new ideas for Western blot normalization that improved the reproducibility of scientific research. We used the Gene Expression Omnibus… read more here.
Keywords: proper loading; loading control; blot normalization; western blot ... See more keywords
Technical Considerations for Contemporary Western Blot Techniques.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1186-9_29
Abstract: Western blotting continues to be a workhorse assay in laboratories throughout the world. The utility, low cost and accessibility of western blotting have allowed the technique to remain in practice, despite being developed over 40 years… read more here.
Keywords: western blot; contemporary western; biology; blot techniques ... See more keywords
Western Blot as a Support Technique for Immunohistochemistry to Detect Programmed Cell Death Ligand 1 Expression.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1278-1_5
Abstract: Antibody selection and optimization are crucial to guarantee accurate and reproducible results when using such antibodies for applications such as western blot analysis and immunohistochemistry (IHC). This is especially important when selecting good candidate antibodies… read more here.
Keywords: technique; immunohistochemistry; western blot; programmed cell ... See more keywords
Western blot analysis of fermented-hydrolyzed foods utilizing gluten-specific antibodies employed in a novel multiplex competitive ELISA
Sign Up to like & getrecommendations! Published in 2019 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-019-01893-0
Abstract: AbstractHorseradish peroxidase (HRP) conjugated gluten-specific antibodies (G12, R5, 2D4, MIoBS, and Skerritt), from nine commercial gluten ELISA test kits, previously utilized in the development of a multiplex competitive ELISA for the detection of fermented-hydrolyzed gluten,… read more here.
Keywords: gluten specific; competitive elisa; western blot; fermented hydrolyzed ... See more keywords
Gastrokine 1 protein is a potential theragnostic target for gastric cancer
Sign Up to like & getrecommendations! Published in 2018 at "Gastric Cancer"
DOI: 10.1007/s10120-018-0828-8
Abstract: BackgroundGastrokine 1 (GKN1) plays important roles in maintaining mucosal homeostasis, and in regulating cell proliferation and differentiation. Here, we determined whether GKN1 is a potential theragnostic marker for gastric cancer.MethodsWe identified GKN1 binding proteins using… read more here.
Keywords: gkn1; gastric cancer; cell proliferation; western blot ... See more keywords
A new background subtraction method for Western blot densitometry band quantification through image analysis software.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of immunological methods"
DOI: 10.1016/j.jim.2018.03.004
Abstract: Since its first description, Western blot has been widely used in molecular labs. It constitutes a multistep method that allows the detection and/or quantification of proteins from simple to complex protein mixtures. Western blot quantification… read more here.
Keywords: analysis; western blot; method; blot ... See more keywords
Quantitative analysis of a biopharmaceutical protein in cell culture samples using automated capillary electrophoresis (CE) western blot
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Pharmaceutical and Biomedical Analysis"
DOI: 10.1016/j.jpba.2017.06.025
Abstract: HighlightsProtein drug titer in cell culture harvest is determined by automated western blot.The distribution of molecular weight isoforms of the protein is measured.Final product quality is predictable from cell culture harvest for process control. Abstract… read more here.
Keywords: analysis; cell culture; cell; western blot ... See more keywords
Yan Y, Ma L, Zhou X, et al. Src inhibition blocks renal interstitial fibroblast activation and ameliorates renal fibrosis. Kidney Int. 2016;89:68-81.
Sign Up to like & getrecommendations! Published in 2017 at "Kidney international"
DOI: 10.1016/j.kint.2017.06.007
Abstract: This article contained errors consisting of incorrect representative photographic and Western blot images. Specifically, the photographic image shown in “ShamþPP1”for p-Histone H3 (Ser 10) duplicated the image of “Sham” in Figure 8c. The Western blot… read more here.
Keywords: image; duplicated image; figure; western blot ... See more keywords
Identification of novel fibronectin-binding proteins by 2D-far Western blot in atypical enteropathogenic Escherichia coli serotype O55:H7.
Sign Up to like & getrecommendations! Published in 2020 at "Microbial pathogenesis"
DOI: 10.1016/j.micpath.2020.104682
Abstract: Atypical enteropathogenic Escherichia coli (aEPEC) is a subgroup of EPEC, which is one of the major pathogens responsible for fatal diarrhoea in children. Compared with typical EPEC (tEPEC), aEPEC lack an EAF (EPEC adherence factor)… read more here.
Keywords: far western; enteropathogenic escherichia; escherichia coli; western blot ... See more keywords
Overcoming off-targets: assessing Western blot signals for Bcnt/Cfdp1, a tentative component of the chromatin remodeling complex
Sign Up to like & getrecommendations! Published in 2020 at "Bioscience Reports"
DOI: 10.1042/bsr20194012
Abstract: Abstract The Bucentaur (BCNT) protein family is characterized by a conserved amino acid sequence at the C-terminus (BCNT-C domain) and plays an essential role in gene expression and chromosomal maintenance in yeast and Drosophila. The… read more here.
Keywords: western blot; bcnt cfdp1; tentative component; bcnt ... See more keywords