Western Blotting with Solutions containing Nanoliter Volumes of Antibody
Sign Up to like & getrecommendations! Published in 2019 at "Current Protocols in Cell Biology"
DOI: 10.1002/cpcb.87
Abstract: Whether screening small mammal serum during antibody production or attempting to preserve a stock of precious antibody, this protocol's western blotting method using aliquots containing nanoliter volumes of antibody will benefit researchers. Time‐tested western blotting… read more here.
Keywords: nanoliter volumes; western blotting; containing nanoliter; solutions containing ... See more keywords
Detection of Post-translationally Modified p53 by Western Blotting.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1217-0_2
Abstract: The p53 tumor suppressor has a central role in many key cellular processes including the DNA damage response, aging, stem cell differentiation, and fertility. p53 undergoes extensive regulatory post-translational modification through events such as phosphorylation,… read more here.
Keywords: post translationally; western blotting; post; p53 ... See more keywords
Exosomes Derived from Senescent Endothelial Cells Contain Distinct Pro-angiogenic miRNAs and Proteins.
Sign Up to like & getrecommendations! Published in 2022 at "Cardiovascular toxicology"
DOI: 10.1007/s12012-022-09740-y
Abstract: Exosomes from senescence cells play pivotal roles in endothelium dysfunction. We investigated the exosomal angiogenic cargo of endothelial cells (ECs) in a model of senescence in vitro. After inducing aging by H2O2, the expression of… read more here.
Keywords: expression; western blotting; mir 126; exosomes derived ... See more keywords
DropBlot: single-cell western blotting of chemically fixed cancer cells
Sign Up to like & getrecommendations! Published in 2024 at "Nature Communications"
DOI: 10.1038/s41467-024-50046-0
Abstract: Archived patient-derived tissue specimens play a central role in understanding disease and developing therapies. To address specificity and sensitivity shortcomings of existing single-cell resolution proteoform analysis tools, we introduce a hybrid microfluidic platform (DropBlot) designed… read more here.
Keywords: retrieval; single cell; chemically fixed; cell ... See more keywords
Utility of a commercial antibody against NTRK1 for western blotting and potential application to immunohistochemistry in adult mouse brain
Sign Up to like & getrecommendations! Published in 2025 at "Scientific Reports"
DOI: 10.1038/s41598-025-88514-2
Abstract: Ntrk1 (also known as TrkA) is a nerve growth factor receptor with essential roles in the development and function of the cholinergic nervous system. Ntrk1 is expressed in a few specific and defined brain areas.… read more here.
Keywords: adult mouse; utility commercial; brain; antibody ... See more keywords
Paeoniflorin inhibits epithelial mesenchymal transformation and oxidative damage of lens epithelial cells in diabetic cataract via sirtuin 1 upregulation
Sign Up to like & getrecommendations! Published in 2022 at "Bioengineered"
DOI: 10.1080/21655979.2021.2018534
Abstract: ABSTRACT Paeoniflorin (Pae) has been reported to serve an important role in complications associated with diabetes. To the best of our knowledge, the role of Pae in diabetic cataracts has not yet been reported. Human… read more here.
Keywords: sra01 cells; damage; lens epithelial; oxidative damage ... See more keywords
Abstract Wed013: The Protective Role of Sirtuin Enzymes in the Development of Cardiac Ischemia in a Rat Model
Sign Up to like & getrecommendations! Published in 2025 at "Circulation Research"
DOI: 10.1161/res.137.suppl_1.wed013
Abstract: Introduction: Sirtuins (SIRT) are a family of enzymes that function as NAD+-dependent deacetylases and ribosyltransferases and are implicated in many biological processes. SIRTs 1/6 have been found to directly influence the pathways that regulate cardiac… read more here.
Keywords: cardiac ischemia; hour; expression sirts; western blotting ... See more keywords
CLDN2 inhibits the metastasis of osteosarcoma cells via down-regulating the afadin/ERK signaling pathway
Sign Up to like & getrecommendations! Published in 2018 at "Cancer Cell International"
DOI: 10.1186/s12935-018-0662-4
Abstract: BackgroundIn an earlier study, we investigated the expression of tight junction protein claudins (CLDNs) in human osteosarcoma (OS) cells, and the CLDN2 was found to be down-regulated in primary tumor cells compared with normal osteoblast cells. Here,… read more here.
Keywords: western blotting; expression; osteosarcoma cells; cldn2 ... See more keywords
Indirubin protects chondrocytes and alleviates OA by inhibiting the MAPK and NF-κB pathways.
Sign Up to like & getrecommendations! Published in 2022 at "International immunopharmacology"
DOI: 10.2139/ssrn.4225767
Abstract: PHARMACOLOGICAL RELEVANCE Indirubin (IR) is a key active ingredient in the traditional Chinese medication QingDai, also called indigo naturalis, which are extensively used in China to treat chronic diseases, such as inflammation and cancer. However,… read more here.
Keywords: chondrocytes alleviates; expression; indirubin protects; protects chondrocytes ... See more keywords
Western blotting: a powerful staple in scientific and biomedical research.
Sign Up to like & getrecommendations! Published in 2022 at "BioTechniques"
DOI: 10.2144/btn-2022-0003
Abstract: Western blotting (WB), also known as immunoblotting, is a well-known molecular biology method that biologists often use to investigate many features of the protein, ranging from basic protein analysis to disease detection. WB is simple,… read more here.
Keywords: scientific biomedical; blotting powerful; western blotting; powerful staple ... See more keywords
Western blotting of native proteins from agarose gels.
Sign Up to like & getrecommendations! Published in 2022 at "BioTechniques"
DOI: 10.2144/btn-2022-0012
Abstract: We have developed a new Western blotting method of native proteins from agarose-based gel electrophoresis using a buffer at pH 6.1 containing basic histidine and acidic 2-(N-morpholino)ethanesulfonic acid. This gel electrophoresis successfully provided native structures… read more here.
Keywords: proteins agarose; native proteins; agarose gels; blotting native ... See more keywords