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   Articles with "whole cell" as a keyword



Whole‐Cell P450 Biocatalysis Using Engineered Escherichia coli with Fine‐Tuned Heme Biosynthesis (Adv. Sci. 6/2023)

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recommendations! Published in 2022 at "Advanced Science"

DOI: 10.1002/advs.202205580

Abstract: By exploiting versatile P450 enzymes, whole‐cell biocatalysis can be performed to synthesize valuable compounds in Escherichia coli. However, the insufficient supply of heme limits the whole‐cell P450 biocatalytic activity. Here a strategy for improving intracellular… read more here.

Keywords: biosynthesis; fine tuned; heme biosynthesis; whole cell ... See more keywords

Design of a cofactor self‐sufficient whole‐cell biocatalyst for enzymatic asymmetric reduction via engineered metabolic pathways and multi‐enzyme cascade

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recommendations! Published in 2024 at "Biotechnology Journal"

DOI: 10.1002/biot.202300744

Abstract: NAD(P)H‐dependent oxidoreductases are crucial biocatalysts for synthesizing chiral compounds. Yet, the industrial implementation of enzymatic redox reactions is often hampered by an insufficient supply of expensive nicotinamide cofactors. Here, a cofactor self‐sufficient whole‐cell biocatalyst was… read more here.

Keywords: cofactor self; whole cell; multi enzyme; cell biocatalyst ... See more keywords

Whole‐cell Escherichia coli lactate biosensor for monitoring mammalian cell cultures during biopharmaceutical production

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recommendations! Published in 2017 at "Biotechnology and Bioengineering"

DOI: 10.1002/bit.26254

Abstract: Many high‐value added recombinant proteins, such as therapeutic glycoproteins, are produced using mammalian cell cultures. In order to optimize the productivity of these cultures it is important to monitor cellular metabolism, for example the utilization… read more here.

Keywords: whole cell; biosensor; mammalian cell; lactate ... See more keywords

Stabilization and scale‐up of photosynthesis‐driven ω‐hydroxylation of nonanoic acid methyl ester by two‐liquid phase whole‐cell biocatalysis

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recommendations! Published in 2019 at "Biotechnology and Bioengineering"

DOI: 10.1002/bit.27006

Abstract: Photoautotrophic organisms are promising hosts for biocatalytic oxyfunctionalizations because they supply reduction equivalents as well as O2 via photosynthetic water oxidation. Thus far, research on photosynthesis‐driven bioprocesses mainly focuses on strain development and the proof… read more here.

Keywords: two liquid; phase; whole cell; liquid phase ... See more keywords

Producing high value unsaturated fatty acid by whole‐cell catalysis using microalga: A case study with Tribonema minus

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recommendations! Published in 2022 at "Biotechnology and Bioengineering"

DOI: 10.1002/bit.28157

Abstract: High value unsaturated fatty acids can be produced by de novo synthesis in microalgal cells, especially via heterotrophic cultivation. Unfortunately, the lipid accumulation of heterotrophic microalgae cannot be improved efficiently in conventional ways. Here we… read more here.

Keywords: high value; value unsaturated; whole cell; acid ... See more keywords

Cellular Biocatalysts Using Synthetic Genetic Circuits for Prolonged and Durable Enzymatic Activity

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recommendations! Published in 2019 at "ChemBioChem"

DOI: 10.1002/cbic.201800767

Abstract: Cellular biocatalysts hold great promise for the synthesis of difficult to achieve compounds, such as complex active molecules. Whole‐cell biocatalysts can be programmed through genetic circuits to be more efficient, but they suffer from low… read more here.

Keywords: genetic circuits; whole cell; biofilm; cellular biocatalysts ... See more keywords

Synthetic Activity of Recombinant Whole Cell Biocatalysts Containing 2‐Deoxy‐D‐ribose‐5‐phosphate Aldolase from Pectobacterium atrosepticum

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recommendations! Published in 2022 at "ChemBioChem"

DOI: 10.1002/cbic.202200147

Abstract: In nature 2‐deoxy‐D‐ribose‐5‐phosphate aldolase (DERA) catalyses the reversible formation of 2‐deoxyribose 5‐phosphate from D‐glyceraldehyde 3‐phosphate and acetaldehyde. In addition, this enzyme can use acetaldehyde as the sole substrate, resulting in a tandem aldol reaction, yielding… read more here.

Keywords: cell; phosphate aldolase; pectobacterium atrosepticum; whole cell ... See more keywords

Whole‐Cell MALDI‐ToF MS Coupled with Untargeted Metabolomics Facilitates Investigations of Microbial Chemical Interactions

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recommendations! Published in 2023 at "ChemBioChem"

DOI: 10.1002/cbic.202200802

Abstract: The emergence of drug‐resistant pathogens necessitates the development of new countermeasures. In this regard, the introduction of probiotics to directly attack or competitively exclude pathogens presents a useful strategy. Application of this approach requires an… read more here.

Keywords: cell; maldi tof; whole cell; cell maldi ... See more keywords

Gram-scale synthesis of (S)-1-(thiophen-2-yl)ethanol in high enantiomeric purity under Enterococcus faecium BY48 biocatalysts.

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recommendations! Published in 2022 at "Chirality"

DOI: 10.1002/chir.23456

Abstract: Sulfur-containing chiral heterocyclic secondary alcohols are relevant intermediates in the preparation of enantiomerically pure compounds endowed with biological activity. In this report, we evaluated the reductive potential of different lactic acid bacteria as whole-cell biocatalysts… read more here.

Keywords: enterococcus faecium; gram scale; by48; whole cell ... See more keywords

Rational Design of Artificial Biofilms as Sustainable Supports for Whole‐Cell Catalysis Through Integrating Extra‐ and Intracellular Catalysis

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recommendations! Published in 2022 at "Chemsuschem"

DOI: 10.1002/cssc.202200850

Abstract: Abstract Biofilms are promising candidates for sustainable bioprocessing applications. This work presents a rational design of biofilm catalysts by integrating extra‐ and intracellular catalysis systems with optimized substrate channeling to realize efficient multistep biosynthesis. An… read more here.

Keywords: catalysis; integrating extra; extra intracellular; whole cell ... See more keywords

Synthesis of 4‐Iodoimidazole Nucleosides: A Biocatalyzed Whole Cell Approach

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recommendations! Published in 2025 at "European Journal of Organic Chemistry"

DOI: 10.1002/ejoc.202400950

Abstract: Nucleoside analogues are biologically active drugs that are well known for their therapeutic properties. Chemical synthesis of these compounds is complex due to time constraints, contaminant methodologies, and numerous steps involved. In contrast, biocatalyzed synthesis,… read more here.

Keywords: whole cell; nucleosides biocatalyzed; synthesis iodoimidazole; biocatalyzed whole ... See more keywords