Whole‐Cell P450 Biocatalysis Using Engineered Escherichia coli with Fine‐Tuned Heme Biosynthesis (Adv. Sci. 6/2023)
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DOI: 10.1002/advs.202205580
Abstract: By exploiting versatile P450 enzymes, whole‐cell biocatalysis can be performed to synthesize valuable compounds in Escherichia coli. However, the insufficient supply of heme limits the whole‐cell P450 biocatalytic activity. Here a strategy for improving intracellular… read more here.
Keywords: biosynthesis; fine tuned; heme biosynthesis; whole cell ... See more keywords
Whole‐cell Escherichia coli lactate biosensor for monitoring mammalian cell cultures during biopharmaceutical production
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26254
Abstract: Many high‐value added recombinant proteins, such as therapeutic glycoproteins, are produced using mammalian cell cultures. In order to optimize the productivity of these cultures it is important to monitor cellular metabolism, for example the utilization… read more here.
Keywords: whole cell; biosensor; mammalian cell; lactate ... See more keywords
Stabilization and scale‐up of photosynthesis‐driven ω‐hydroxylation of nonanoic acid methyl ester by two‐liquid phase whole‐cell biocatalysis
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.27006
Abstract: Photoautotrophic organisms are promising hosts for biocatalytic oxyfunctionalizations because they supply reduction equivalents as well as O2 via photosynthetic water oxidation. Thus far, research on photosynthesis‐driven bioprocesses mainly focuses on strain development and the proof… read more here.
Keywords: two liquid; phase; whole cell; liquid phase ... See more keywords
Producing high value unsaturated fatty acid by whole‐cell catalysis using microalga: A case study with Tribonema minus
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DOI: 10.1002/bit.28157
Abstract: High value unsaturated fatty acids can be produced by de novo synthesis in microalgal cells, especially via heterotrophic cultivation. Unfortunately, the lipid accumulation of heterotrophic microalgae cannot be improved efficiently in conventional ways. Here we… read more here.
Keywords: high value; value unsaturated; whole cell; acid ... See more keywords
Cellular Biocatalysts Using Synthetic Genetic Circuits for Prolonged and Durable Enzymatic Activity
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DOI: 10.1002/cbic.201800767
Abstract: Cellular biocatalysts hold great promise for the synthesis of difficult to achieve compounds, such as complex active molecules. Whole‐cell biocatalysts can be programmed through genetic circuits to be more efficient, but they suffer from low… read more here.
Keywords: genetic circuits; whole cell; biofilm; cellular biocatalysts ... See more keywords
Synthetic Activity of Recombinant Whole Cell Biocatalysts Containing 2‐Deoxy‐D‐ribose‐5‐phosphate Aldolase from Pectobacterium atrosepticum
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DOI: 10.1002/cbic.202200147
Abstract: In nature 2‐deoxy‐D‐ribose‐5‐phosphate aldolase (DERA) catalyses the reversible formation of 2‐deoxyribose 5‐phosphate from D‐glyceraldehyde 3‐phosphate and acetaldehyde. In addition, this enzyme can use acetaldehyde as the sole substrate, resulting in a tandem aldol reaction, yielding… read more here.
Keywords: cell; phosphate aldolase; pectobacterium atrosepticum; whole cell ... See more keywords
Whole‐Cell MALDI‐ToF MS Coupled with Untargeted Metabolomics Facilitates Investigations of Microbial Chemical Interactions
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DOI: 10.1002/cbic.202200802
Abstract: The emergence of drug‐resistant pathogens necessitates the development of new countermeasures. In this regard, the introduction of probiotics to directly attack or competitively exclude pathogens presents a useful strategy. Application of this approach requires an… read more here.
Keywords: cell; maldi tof; whole cell; cell maldi ... See more keywords
Gram-scale synthesis of (S)-1-(thiophen-2-yl)ethanol in high enantiomeric purity under Enterococcus faecium BY48 biocatalysts.
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DOI: 10.1002/chir.23456
Abstract: Sulfur-containing chiral heterocyclic secondary alcohols are relevant intermediates in the preparation of enantiomerically pure compounds endowed with biological activity. In this report, we evaluated the reductive potential of different lactic acid bacteria as whole-cell biocatalysts… read more here.
Keywords: enterococcus faecium; gram scale; by48; whole cell ... See more keywords
Rational Design of Artificial Biofilms as Sustainable Supports for Whole‐Cell Catalysis Through Integrating Extra‐ and Intracellular Catalysis
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DOI: 10.1002/cssc.202200850
Abstract: Abstract Biofilms are promising candidates for sustainable bioprocessing applications. This work presents a rational design of biofilm catalysts by integrating extra‐ and intracellular catalysis systems with optimized substrate channeling to realize efficient multistep biosynthesis. An… read more here.
Keywords: catalysis; integrating extra; extra intracellular; whole cell ... See more keywords
Optomechanical measurement of the role of lamins in whole cell deformability.
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DOI: 10.1002/jbio.201600198
Abstract: There is mounting evidence that the nuclear envelope, and particularly the lamina, plays a critical role in the mechanical and regulation properties of the cell and changes to the lamina can have implications for the… read more here.
Keywords: whole cell; optical stretcher; role; deformability ... See more keywords
Whole-Cell Biocatalysis in Ionic Liquids.
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DOI: 10.1007/10_2018_77
Abstract: The use of whole-cell biocatalysis in ionic liquid (IL)-containing systems has attracted increasing attention in recent years. Compared to bioreactions catalyzed by isolated enzymes, the major advantage of using whole cells in biocatalytic processes is… read more here.
Keywords: whole cell; cell biocatalysis; ionic liquids; cell ... See more keywords