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   Articles with "whole cells" as a keyword



Bio‐preparation of (R)‐DMPM using whole cells of Pseudochrobactrum asaccharolyticum WZZ003 and its application on kilogram‐scale synthesis of fungicide (R)‐metalaxyl

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recommendations! Published in 2018 at "Biotechnology Progress"

DOI: 10.1002/btpr.2638

Abstract: Methyl (R)‐N‐(2,6‐dimethylphenyl)alaninate ((R)‐DMPM) is a key chiral intermediate for the production of (R)‐metalaxyl, which is one of the best‐selling fungicides. A new strain, Pseudochrobactrum asaccharolyticum WZZ003, was identified as a biocatalyst for the enantioselective hydrolysis… read more here.

Keywords: dmpm; pseudochrobactrum asaccharolyticum; asaccharolyticum wzz003; kilogram scale ... See more keywords
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Stereoselective synthesis of a key chiral intermediate of (S)-Rivastigmine by AKR-GDH recombinant whole cells.

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recommendations! Published in 2019 at "Journal of biotechnology"

DOI: 10.1016/j.jbiotec.2018.11.016

Abstract: Herein, three aldo/ketoreductases (AKRs) were obtained and used to prepare N- ethyl-methyl-carbamic acid-3-[(1S)-hydroxy-ethyl]-phenyl ester ((S)-NEMCA-HEPE), which is a key intermediate of (S)-Rivastigmine. To avoid the usage of extra cofactors, the recombinant whole-cells containing AKRs and… read more here.

Keywords: gdh; reaction; recombinant whole; intermediate rivastigmine ... See more keywords
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Enzyme Cascades in Whole Cells for the Synthesis of Chiral Cyclic Amines

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recommendations! Published in 2017 at "ACS Catalysis"

DOI: 10.1021/acscatal.7b00513

Abstract: The increasing diversity of reactions mediated by biocatalysts has led to development of multistep in vitro enzyme cascades, taking advantage of generally compatible reaction conditions. The construction of pathways within single whole cell systems is… read more here.

Keywords: enzyme cascades; cells synthesis; whole cell; synthesis chiral ... See more keywords
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A Fluorescent Probe Distinguishes between Inhibition of Early and Late Steps of Lipopolysaccharide Biogenesis in Whole Cells.

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recommendations! Published in 2017 at "ACS chemical biology"

DOI: 10.1021/acschembio.7b00159

Abstract: Lipopolysaccharide (LPS) biogenesis in Gram-negative organisms involves its biosynthesis in the cytoplasm and subsequent transport across three cellular compartments to the cell surface. We developed a fluorescent probe that allows us to determine the spatial… read more here.

Keywords: distinguishes inhibition; fluorescent probe; probe distinguishes; early late ... See more keywords
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Rapid and simple purification of elastin-like polypeptides directly from whole cells and cell lysates by organic solvent extraction.

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recommendations! Published in 2018 at "Biomaterials science"

DOI: 10.1039/c8bm00124c

Abstract: Elastin-like polypeptides (ELP) are a well-known class of proteins that are being increasingly utilized in a variety of biomedical applications, due to their beneficial physicochemical properties. A unifying feature of ELP is their demonstration of… read more here.

Keywords: extraction; like polypeptides; elp; elastin like ... See more keywords
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Rapid conversion of cyclohexenone, cyclohexanone and cyclohexanol to ε-caprolactone by whole cells of Geotrichum candidum CCT 1205

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recommendations! Published in 2017 at "Biocatalysis and Biotransformation"

DOI: 10.1080/10242422.2017.1310207

Abstract: Abstract ɛ-Caprolactone (ɛ-CL) was obtained with excellent conversion and short reaction times from the substrates cyclohexenone, cyclohexanone and cyclohexanol using whole cells of Brazilian Geotrichum candidum (CCT 1205). The reactions were monitored over time by… read more here.

Keywords: cyclohexenone cyclohexanone; cyclohexanone cyclohexanol; whole cells; geotrichum candidum ... See more keywords
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Enhanced electrochemical measurement of β-galactosidase activity in whole cells by coexpression of lactose permease, LacY.

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recommendations! Published in 2022 at "BioTechniques"

DOI: 10.2144/btn-2022-0090

Abstract: Whole-cell biosensing links the sensing and computing capabilities of microbes to the generation of a detectable reporter. Whole cells enable dynamic biological computation (filtered noise, amplified signals, logic gating etc.). Enzymatic reporters enable in situ… read more here.

Keywords: lacy; coexpression lactose; lactose permease; whole cells ... See more keywords
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Rapid Detection of Lipopolysaccharide and Whole Cells of Francisella tularensis Based on Agglutination of Antibody-Coated Gold Nanoparticles and Colorimetric Registration

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recommendations! Published in 2022 at "Micromachines"

DOI: 10.3390/mi13122194

Abstract: The paper presents development and characterization of a new bioanalytical test system for rapid detection of lipopolysaccharide (LPS) and whole cells of Francisella tularensis, a causative agent of tularemia, in water samples. Gold nanoparticles (AuNPs)… read more here.

Keywords: cells francisella; detection lipopolysaccharide; tularensis; rapid detection ... See more keywords