Ex Vivo Whole-Mount Imaging of Leukocyte Migration to the Bone Marrow.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1425-9_12
Abstract: The bone marrow is the major hematopoietic organ, consisting of distinct microenvironmental niches for the production of hematopoietic cells. Advanced visualizing methods are required to define and better understand the interactions between stromal and hematopoietic… read more here.
Keywords: whole mount; mount imaging; bone; vivo whole ... See more keywords
Added Clinical Value of Whole-mount Histopathology of Radical Prostatectomy Specimens: A Collaborative Review.
Sign Up to like & getrecommendations! Published in 2020 at "European urology oncology"
DOI: 10.1016/j.euo.2020.08.003
Abstract: CONTEXT Whole-mount histopathology, that is, large format histology or whole-mount sectioning, refers to the histopathological examination of tissue sections from specimens processed with large tissue cassette. In some institutions, it is applied routinely to specimens… read more here.
Keywords: whole mount; mount histopathology; histopathology; radical prostatectomy ... See more keywords
Fix the problem – A practical guide to whole-mount immunohistochemistry of teased nerve fibres
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Neuroscience Methods"
DOI: 10.1016/j.jneumeth.2017.06.019
Abstract: BACKGROUND Immunohistochemical staining of entire nerve fibres allows for studying the molecular composition of functional fibre subunits and may add to the diagnostic value of nerve fibre teasing. NEW METHOD In this study, we established… read more here.
Keywords: whole mount; teased nerve; fix problem; nerve fibres ... See more keywords
Whole-mount Dual Labeling of Regenerating Bone and Vasculature in Axolotl Appendages
Sign Up to like & getrecommendations! Published in 2018 at "Microscopy and Microanalysis"
DOI: 10.1017/s1431927618007079
Abstract: Bone is a highly vascular tissue and angiogenesis, the formation of new capillaries from pre-existing vessels, plays a pivotal role in skeletal growth. Therapies that increase angiogenesis have been shown to improve bone repair (e.g.,… read more here.
Keywords: whole mount; angiogenesis; vasculature; tissue ... See more keywords
Whole-Mount Adult Ear Skin Imaging Reveals Defective Neuro-Vascular Branching Morphogenesis in Obese and Type 2 Diabetic Mouse Models
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-18581-7
Abstract: Obesity and type 2 diabetes are frequently associated with peripheral neuropathy. Though there are multiple methods for diagnosis and analysis of morphological changes of peripheral nerves and blood vessels, three-dimensional high-resolution imaging is necessary to… read more here.
Keywords: ear skin; branching morphogenesis; whole mount; mouse ... See more keywords
Immunofluorescent Staining of Whole-Mount Mouse Embryos.
Sign Up to like & getrecommendations! Published in 2018 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot094037
Abstract: Mouse embryos and fetal organs have no pigmentation except for the retinal pigmented epithelium of the eye at subsequent stages of development. This makes it difficult to visualize and photodocument embryonic structures using conventional light… read more here.
Keywords: whole mount; microscopy; staining whole; mouse embryos ... See more keywords
Immunostaining Whole-Mount Olfactory Tissues of Mosquitoes.
Sign Up to like & getrecommendations! Published in 2022 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot107915
Abstract: Mosquito olfactory tissues are covered in fine hair-like sensory structures called sensilla that house olfactory sensory neurons. This protocol for immunostaining whole-mounted mosquito antennae and maxillary palps enables the visualization of these neurons. read more here.
Keywords: mount olfactory; immunostaining whole; whole mount; olfactory tissues ... See more keywords
RNA In Situ Hybridization of Whole-Mount Mosquito Olfactory Tissues.
Sign Up to like & getrecommendations! Published in 2022 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot107916
Abstract: This protocol presents a method for RNA in situ hybridization of whole-mounted preparations of mosquito antennae and maxillary palps. It can be adapted for other peripheral appendages such as the legs or proboscis. read more here.
Keywords: mount mosquito; situ hybridization; hybridization whole; rna situ ... See more keywords
Abstract 140: Clonal evaluation of ERG/SPINK1 status to determine disease progression using whole mount prostatectomy specimens
Sign Up to like & getrecommendations! Published in 2023 at "Cancer Research"
DOI: 10.1158/1538-7445.am2023-140
Abstract: Most primary prostate cancers are multifocal, marked by distinct tumor foci. Multiclonality is not routinely incorporated at clinical evaluation, however, marker status as a pathological parameter holds great potential for improving prognostic accuracy. Here, we… read more here.
Keywords: erg spink1; whole mount; spink1; status ... See more keywords
Whole-mount in situ hybridization: minimizing the folding problem of thin-sheet tissue-like crayfish haematopoietic tissue
Sign Up to like & getrecommendations! Published in 2018 at "Crustaceana"
DOI: 10.1163/15685403-00003745
Abstract: Crayfish haematopoietic tissue (HPT) has a thin-sheet-like structure with a thickness of 100-160 μ m and a width of approximately 1-2 cm. This structure makes HPT extremely easy to fold after removal from the animal. Therefore,… read more here.
Keywords: whole mount; tissue; thin sheet; crayfish haematopoietic ... See more keywords
All-age whole mount in situ hybridization to reveal larval and juvenile expression patterns in zebrafish
Sign Up to like & getrecommendations! Published in 2020 at "PLoS ONE"
DOI: 10.1371/journal.pone.0237167
Abstract: The zebrafish Danio rerio is a valuable and common model for scientists in the fields of genetics and developmental biology. Since zebrafish are also amenable to genetic manipulation, modelling of human diseases or behavioral experiments… read more here.
Keywords: whole mount; mount situ; gene expression; expression ... See more keywords