Genome‐wide CRISPR‐Cas9 screen reveals a persistent null‐hyphal phenotype that maintains high carotenoid production in Yarrowia lipolytica
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28219
Abstract: Yarrowia lipolytica is a metabolic engineering host of growing industrial interest due to its ability to metabolize hydrocarbons, fatty acids, glycerol, and other renewable carbon sources. This dimorphic yeast undergoes a stress‐induced transition to a… read more here.
Keywords: screen; genome wide; yarrowia lipolytica; production ... See more keywords
Next-Generation Sequencing of Genome-Wide CRISPR Screens.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7514-3_13
Abstract: Genome-wide functional genomic screens utilizing the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system have proven to be a powerful tool for systematic genomic perturbation in mammalian cells and provide an alternative to previous screens… read more here.
Keywords: genome wide; next generation; generation sequencing; wide crispr ... See more keywords
Genome-Wide CRISPR/Cas9 Screening for Identification of Cancer Genes in Cell Lines.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8967-6_10
Abstract: In this protocol, pooled sgRNA libraries targeting thousands of genes are computationally designed, generated using microarray-based synthesis techniques, and packaged into lentiviral particles. Target cells of interest are transduced with the lentiviral sgRNA pools to… read more here.
Keywords: identification cancer; genome wide; cas9; wide crispr ... See more keywords
A Genome-wide CRISPR Screen Identifies ZCCHC14 as a Host Factor Required for Hepatitis B Surface Antigen Production.
Sign Up to like & getrecommendations! Published in 2019 at "Cell reports"
DOI: 10.1016/j.celrep.2019.10.113
Abstract: A hallmark of chronic hepatitis B (CHB) virus infection is the presence of high circulating levels of non-infectious small lipid HBV surface antigen (HBsAg) vesicles. Although rare, sustained HBsAg loss is the idealized endpoint of… read more here.
Keywords: genome wide; surface antigen; hepatitis; wide crispr ... See more keywords
Genome-wide CRISPR-Cas9 Interrogation of Splicing Networks Reveals a Mechanism for Recognition of Autism-Misregulated Neuronal Microexons.
Sign Up to like & getrecommendations! Published in 2018 at "Molecular cell"
DOI: 10.1016/j.molcel.2018.10.008
Abstract: Alternative splicing is crucial for diverse cellular, developmental, and pathological processes. However, the full networks of factors that control individual splicing events are not known. Here, we describe a CRISPR-based strategy for the genome-wide elucidation… read more here.
Keywords: genome wide; mechanism; autism; wide crispr ... See more keywords
Genome-wide CRISPR-Cas9 screening in mammalian cells.
Sign Up to like & getrecommendations! Published in 2019 at "Methods"
DOI: 10.1016/j.ymeth.2019.04.015
Abstract: Forward genetic screens are a powerful and unbiased approach for uncovering the genetic basis behind a specific phenotype. Genome-wide mutagenesis followed by phenotypic screening represents the ultimate manifestation of this method, directly linking biological phenomena… read more here.
Keywords: genome wide; cas9 screening; crispr; crispr cas9 ... See more keywords
Genome-wide CRISPR Screening to Identify Drivers of TGF-β-Induced Liver Fibrosis in Human Hepatic Stellate Cells
Sign Up to like & getrecommendations! Published in 2022 at "ACS Chemical Biology"
DOI: 10.1021/acschembio.2c00006
Abstract: Liver fibrosis progression in chronic liver disease leads to cirrhosis, liver failure, or hepatocellular carcinoma and often ends in liver transplantation. Even with an increased understanding of liver fibrogenesis and many attempts to generate therapeutics… read more here.
Keywords: liver fibrosis; tgf induced; genome wide; wide crispr ... See more keywords
Genome-wide CRISPR screens reveal a Wnt–FZD5 signaling circuit as a druggable vulnerability of RNF43-mutant pancreatic tumors
Sign Up to like & getrecommendations! Published in 2017 at "Nature Medicine"
DOI: 10.1038/nm.4219
Abstract: Forward genetic screens with CRISPR–Cas9 genome editing enable high-resolution detection of genetic vulnerabilities in cancer cells. We conducted genome-wide CRISPR–Cas9 screens in RNF43-mutant pancreatic ductal adenocarcinoma (PDAC) cells, which rely on Wnt signaling for proliferation.… read more here.
Keywords: rnf43 mutant; mutant pancreatic; genome wide; wide crispr ... See more keywords
Corrigendum: Genome-wide CRISPR screens reveal a Wnt–FZD5 signaling circuit as a druggable vulnerability of RNF43-mutant pancreatic tumors
Sign Up to like & getrecommendations! Published in 2017 at "Nature Medicine"
DOI: 10.1038/nm1117-1384d
Abstract: This corrects the article DOI: 10.1038/nm.4219 read more here.
Keywords: reveal wnt; genome wide; wide crispr; corrigendum genome ... See more keywords
Genome-wide CRISPR-Cas9 Screening Reveals Ubiquitous T cell Cancer Targeting via the monomorphic MHC class I related protein MR1
Sign Up to like & getrecommendations! Published in 2020 at "Nature immunology"
DOI: 10.1038/s41590-019-0578-8
Abstract: Human leukocyte antigen (HLA)-independent, T cell–mediated targeting of cancer cells would allow immune destruction of malignancies in all individuals. Here, we use genome-wide CRISPR–Cas9 screening to establish that a T cell receptor (TCR) recognized and… read more here.
Keywords: genome wide; cas9 screening; crispr cas9; wide crispr ... See more keywords
A genome-wide CRISPR/Cas9 screen to identify phagocytosis modulators in monocytic THP-1 cells
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-92332-7
Abstract: Phagocytosis of microbial pathogens, dying or dead cells, and cell debris is essential to maintain tissue homeostasis. Impairment of these processes is associated with autoimmunity, developmental defects and toxic protein accumulation. However, the underlying molecular… read more here.
Keywords: genome wide; phagocytosis; wide crispr; monocytic thp ... See more keywords