Bisulfite Sequencing of RNA for Transcriptome-Wide Detection of 5-Methylcytosine.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8808-2_1
Abstract: A powerful method to determine the methylation status of specific cytosine residues within RNA is bisulfite sequencing. In combination with high-throughput sequencing methods cytosine methylation can be determined at nucleotide resolution on a transcriptome-wide level.… read more here.
Keywords: sequencing rna; bisulfite sequencing; rna transcriptome; wide detection ... See more keywords
Genome-wide detection of human variants that disrupt intronic branchpoints
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1101/2022.04.18.488668
Abstract: Pre-mRNA splicing is initiated with the recognition of a single-nucleotide intronic branchpoint (BP) within a BP motif by spliceosome elements. Fifty-six rare variants in 44 human genes have been reported to alter splicing and cause… read more here.
Keywords: detection human; human variants; genome wide; variants disrupt ... See more keywords