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Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1851-6_17
Abstract: Gene expression is controlled at multiple levels, including RNA transcription and turnover. But determining the relative contributions of RNA biogenesis and decay to the steady-state abundance of cellular transcripts remains challenging because conventional transcriptomics approaches…
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Keywords:
profiling rna;
rna stability;
transcriptome wide;
wide profiling ... See more keywords
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Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9865-4_9
Abstract: Chromatin immunoprecipitation coupled with sequencing (ChIP-seq) is a widely used method for mapping the genome-wide locations of chromatin-associated proteins. This protocol has been developed and utilized to perform ChIP on histone covalent modifications in various…
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Keywords:
chip seq;
genome wide;
wide profiling;
histone ... See more keywords
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Published in 2017 at "Methods"
DOI: 10.1016/j.ymeth.2017.06.003
Abstract: Alternative polyadenylation (APA) diversifies the 3' termini of a majority of mRNAs in most eukaryotes, and is consequently inferred to have substantial consequences for the utilization of post-transcriptional regulatory mechanisms. Since conventional RNA-sequencing methods do…
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Keywords:
genome wide;
profiling ends;
polyadenylated rnas;
ends polyadenylated ... See more keywords
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Published in 2017 at "PLoS ONE"
DOI: 10.1371/journal.pone.0174865
Abstract: Background Cancers of the oral cavity are primarily oral squamous cell carcinomas (OSCCs). Many of the OSCCs present at late stages with an exceptionally poor prognosis. A probable limitation in management of patients with OSCC…
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Keywords:
genome wide;
chromosomal regions;
oral squamous;
squamous cell ... See more keywords
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1
Published in 2022 at "Life Science Alliance"
DOI: 10.26508/lsa.202201778
Abstract: We present a detailed step-wise protocol for reliable genome-wide profiling of histone modifications using CUT&RUN that requires only low numbers of Plasmodium falciparum blood stages. We recently adapted a CUT&RUN protocol for genome-wide profiling of…
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Keywords:
histone modifications;
cut run;
genome wide;
wide profiling ... See more keywords
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Published in 2017 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms18122599
Abstract: Auxin has long been known as a critical phytohormone that regulates fruit development in plants. However, due to the lack of an enlarged ovary wall in the model plants Arabidopsis and rice, the molecular regulatory…
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Keywords:
genome wide;
fruit;
genes fruit;
profiling small ... See more keywords