An improved strategy for CRISPR/Cas9 gene knockout and subsequent wildtype and mutant gene rescue
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DOI: 10.1371/journal.pone.0228910
Abstract: A fluorescence marker mOrange was inserted to the popular pLentiCrispr-V2 to create pLentiCrispr-V2-mOrange (V2mO) that contained both a puromycin selection and a fluorescent marker, making viral production and target transduction visible. Lentiviruses packaged with this… read more here.
Keywords: crispr cas9; target; wildtype mutant; improved strategy ... See more keywords