Human Protein‐Based Porous Scaffolds as Platforms for Xeno‐Free 3D Cell Culture
Sign Up to like & getrecommendations! Published in 2022 at "Advanced Healthcare Materials"
DOI: 10.1002/adhm.202102383
Abstract: Extracellular matrix and protein‐based biomaterials emerge as attractive sources to produce scaffolds due to their great properties regarding biocompatibility and bioactivity. In addition, there are concerns regarding the use of animal‐derived supplements in cell culture… read more here.
Keywords: culture; cell culture; xeno free; protein based ... See more keywords
Human Albumin-Based Hydrogels for Their Potential Xeno-Free Microneedle Applications.
Sign Up to like & getrecommendations! Published in 2022 at "Macromolecular bioscience"
DOI: 10.1002/mabi.202200463
Abstract: Nowadays, hydrogels-based microneedles (MNs) have attracted a great interest owing to their outstanding qualities for biomedical applications. For the fabrication of hydrogels-based microneedles as tissue engineering scaffolds and drug delivery carriers, various biomaterials have been… read more here.
Keywords: xeno free; based hydrogels; human albumin; albumin based ... See more keywords
Recombinant human type II collagen hydrogel provides a xeno‐free 3D micro‐environment for chondrogenesis of human bone marrow‐derived mesenchymal stromal cells
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Tissue Engineering and Regenerative Medicine"
DOI: 10.1002/term.1983
Abstract: Recombinant human type II collagen (rhCII) hydrogel was tested as a xeno‐free micro‐environment for the chondrogenesis of human bone marrow‐derived mesenchymal stromal cells (BM‐MSCs). The rhCII hydrogels were seeded with BM‐MSCs and cultured in a… read more here.
Keywords: collagen; recombinant human; hydrogel; chondrogenesis ... See more keywords
CRISPR/Cas9-Mediated Mutagenesis of Human Pluripotent Stem Cells in Defined Xeno-Free E8 Medium.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6472-7_5
Abstract: The recent advent of engineered nucleases including the CRISPR/Cas9 system has greatly facilitated genome manipulation in human pluripotent stem cells (hPSCs). In addition to facilitating hPSC-based disease studies, the application of genome engineering in hPSCs… read more here.
Keywords: crispr cas9; xeno free; defined xeno;
Xeno-free approach for the expansion of human adipose derived mesenchymal stem cells for ocular therapies.
Sign Up to like & getrecommendations! Published in 2020 at "Experimental eye research"
DOI: 10.1016/j.exer.2020.108358
Abstract: To restore corneal transparency and vision loss after an injury on the ocular surface, the use of human stem cells from different origins has been recently proposed. Mesenchymal stem cells (MSCs) seem to be an… read more here.
Keywords: expansion; xeno free; stem; stem cells ... See more keywords
Establishing iPSC cell banks derived using reagents and workflows optimized for cell therapy manufacturing
Sign Up to like & getrecommendations! Published in 2019 at "Cytotherapy"
DOI: 10.1016/j.jcyt.2019.03.574
Abstract: Background & Aim Induced pluripotent stem cell (iPSC) research is rapidly moving towards translational and clinical applications.These applications require robust and consistent workflows that utilize high quality reagents that are xeno-free.When commonly used animal-origin components… read more here.
Keywords: quality; establishing ipsc; xeno free; donor ... See more keywords
Generation of nonviral integration-free human iPS cell line KISCOi001-A from normal human fibroblasts, under defined xeno-free and feeder-free conditions.
Sign Up to like & getrecommendations! Published in 2021 at "Stem cell research"
DOI: 10.1016/j.scr.2021.102193
Abstract: KISCOi001-A is a healthy feeder-free and fully characterized human induced pluripotent stem (iPS) cell line cultured under xeno-free and defined conditions. The cell line is generated from normal human foreskin fibroblasts with non-integrating episomal plasmid… read more here.
Keywords: cell line; line; feeder free; ips cell ... See more keywords
Xeno-free pre-vascularized spheroids for therapeutic applications
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-18431-6
Abstract: Spheroid culture has gained increasing popularity, arising as a promising tool for regenerative medicine applications. Importantly, spheroids may present advantages over single-cell suspensions in cell-based therapies (CT). Unfortunately, most growth media used for spheroid culture… read more here.
Keywords: therapeutic applications; pre vascularized; xeno free; vascularized spheroids ... See more keywords
Xeno-free bioengineered human skeletal muscle tissue using human platelet lysate-based hydrogels
Sign Up to like & getrecommendations! Published in 2022 at "Biofabrication"
DOI: 10.1088/1758-5090/ac8dc8
Abstract: Bioengineered human skeletal muscle tissues have emerged in the last years as new in vitro systems for disease modeling. These bioartificial muscles are classically fabricated by encapsulating human myogenic precursor cells in a hydrogel scaffold… read more here.
Keywords: bioengineered human; skeletal muscle; xeno free; human skeletal ... See more keywords
A new clinical‐scale serum‐free xeno‐free medium efficient in ex vivo amplification of mesenchymal stromal cells does not support mesenchymal stem cells
Sign Up to like & getrecommendations! Published in 2017 at "Transfusion"
DOI: 10.1111/trf.13902
Abstract: We evaluated a new serum‐free, xeno‐free medium (Xuri, GE HealthCare) in ex vivo cultures for amplification of mesenchymal stromal cells (MStroC) in comparison with classical culture supplemented with fetal calf serum and basic fibroblast growth… read more here.
Keywords: mesenchymal stromal; free medium; free xeno; xeno free ... See more keywords
A Xeno-Free Strategy for Derivation of Human Umbilical Vein Endothelial Cells and Wharton's Jelly Derived Mesenchymal Stromal Cells: A Feasibility Study toward Personal Cell and Vascular Based Therapy
Sign Up to like & getrecommendations! Published in 2020 at "Stem Cells International"
DOI: 10.1155/2020/8832052
Abstract: Coimplantation of endothelial cells (ECs) and mesenchymal stromal cells (MSCs) into the transplantation site could be a feasible option to achieve a sufficient level of graft-host vascularization. To find a suitable source of tissue that… read more here.
Keywords: mesenchymal stromal; stromal cells; free strategy; xeno free ... See more keywords