Automated multi‐sample DNA extraction for genotyping live Xenopus embryos
Sign Up to like & getrecommendations! Published in 2022 at "Developmental Dynamics"
DOI: 10.1002/dvdy.544
Abstract: Xenopus frogs are used extensively for modeling genetic diseases owing to characteristics such as the abundance of eggs combined with their large size, allowing easy manipulation, and rapid external embryo development enabling the examination of… read more here.
Keywords: multi sample; dna extraction; automated multi; xenopus ... See more keywords
Maternal messages to live by: a personal historical perspective
Sign Up to like & getrecommendations! Published in 2017 at "genesis"
DOI: 10.1002/dvg.23007
Abstract: In the 1980s, the study of localized maternal mRNAs was just emerging as a new research area. Classic embryological studies had linked the inheritance of cytoplasmic domains with specific cell lineages, but the underlying molecular… read more here.
Keywords: xenopus; personal historical; historical perspective; maternal messages ... See more keywords
Husbandry, General Care, and Transportation of Xenopus laevis and Xenopus tropicalis.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8784-9_1
Abstract: Maintenance of optimal conditions such as water parameters, diet, and feeding is essential to a healthy Xenopus laevis and Xenopus tropicalis colony and thus to the productivity of the lab. Our prior husbandry experience as… read more here.
Keywords: xenopus; laevis xenopus; xenopus laevis; xenopus tropicalis ... See more keywords
Xenopus extract approaches to studying microtubule organization and signaling in cytokinesis.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in cell biology"
DOI: 10.1016/bs.mcb.2016.04.014
Abstract: We report optimized methods for preparing actin-intact Xenopus egg extract. This extract is minimally perturbed, undiluted egg cytoplasm where the cell cycle can be experimentally controlled. It contains abundant organelles and glycogen and supports active metabolism… read more here.
Keywords: xenopus; microtubule organization; actin intact; egg ... See more keywords
Bisphenols disrupt thyroid hormone (TH) signaling in the brain and affect TH-dependent brain development in Xenopus laevis.
Sign Up to like & getrecommendations! Published in 2021 at "Aquatic toxicology"
DOI: 10.1016/j.aquatox.2021.105902
Abstract: There is concern about adverse effects of thyroid hormone (TH) disrupting chemicals on TH-dependent brain development. Bisphenol A (BPA) and its analogues, such as bisphenol F (BPF), are known to have the potential to interfere… read more here.
Keywords: xenopus; dependent brain; brain development; brain ... See more keywords
Gli2 is required for the induction and migration of Xenopus laevis neural crest
Sign Up to like & getrecommendations! Published in 2018 at "Mechanisms of Development"
DOI: 10.1016/j.mod.2018.07.010
Abstract: The neural crest (NC) is a multipotent migratory embryonic population that is formed during late gastrulation and gives rise to a wide array of derivatives, including cells from the peripheral nervous system (PNS), the craniofacial… read more here.
Keywords: xenopus; gli2 required; neural crest; required induction ... See more keywords
Developmental neurogenesis in mouse and Xenopus is impaired in the absence of Nosip.
Sign Up to like & getrecommendations! Published in 2017 at "Developmental biology"
DOI: 10.1016/j.ydbio.2017.06.026
Abstract: BACKGROUND Genetic deletion of Nosip in mice causes holoprosencephaly, however, the function of Nosip in neurogenesis is currently unknown. RESULTS We combined two vertebrate model organisms, the mouse and the South African clawed frog, Xenopus… read more here.
Keywords: xenopus; neurogenesis; nosip; mouse xenopus ... See more keywords
Role of maternal Xenopus syntabulin in germ plasm aggregation and primordial germ cell specification.
Sign Up to like & getrecommendations! Published in 2017 at "Developmental biology"
DOI: 10.1016/j.ydbio.2017.10.006
Abstract: The localization and organization of mitochondria- and ribonucleoprotein granule-rich germ plasm is essential for many aspects of germ cell development. In Xenopus, germ plasm is maternally inherited and is required for the specification of primordial… read more here.
Keywords: xenopus; plasm aggregation; germ; germ plasm ... See more keywords
Lipid Metabolic Disorder Induced by Pyrethroids in Nonalcoholic Fatty Liver Disease of Xenopus laevis.
Sign Up to like & getrecommendations! Published in 2022 at "Environmental science & technology"
DOI: 10.1021/acs.est.2c00516
Abstract: Pyrethroids, an effective and widely used class of pesticides, have attracted considerable concerns considering their frequent detection in environmental matrices. However, their potential health risks to amphibians remain unclear. In our study, female Xenopus laevis… read more here.
Keywords: nonalcoholic fatty; xenopus laevis; cis; metabolic disorder ... See more keywords
Identification of novel cis-regulatory elements of Eya1 in Xenopus laevis using BAC recombineering
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-15153-7
Abstract: The multifunctional Eya1 protein plays important roles during the development of cranial sensory organs and ganglia, kidneys, hypaxial muscles and several other organs in vertebrates. Eya1 is encoded by a complex locus with candidate cis-regulatory… read more here.
Keywords: xenopus; bac recombineering; xenopus laevis; enhancer activity ... See more keywords
Xenbase: key features and resources of the Xenopus model organism knowledgebase
Sign Up to like & getrecommendations! Published in 2023 at "Genetics"
DOI: 10.1093/genetics/iyad018
Abstract: Abstract Xenbase (https://www.xenbase.org/), the Xenopus model organism knowledgebase, is a web-accessible resource that integrates the diverse genomic and biological data from research on the laboratory frogs Xenopus laevis and Xenopus tropicalis. The goal of Xenbase… read more here.
Keywords: xenopus model; ontology; xenbase; model organism ... See more keywords