Quantitative Proteomics of Xenopus Embryos I, Sample Preparation.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8784-9_13
Abstract: Xenopus oocytes and embryos are model systems optimally suited for quantitative proteomics. This is due to the availability of large amount of protein material and the ease of physical manipulation. Furthermore, facile in vitro fertilization… read more here.
Keywords: xenopus embryos; proteomics xenopus; sample preparation; quantitative proteomics ... See more keywords
E-liquids and vanillin flavoring disrupts retinoic acid signaling and causes craniofacial defects in Xenopus embryos.
Sign Up to like & getrecommendations! Published in 2021 at "Developmental biology"
DOI: 10.1016/j.ydbio.2021.09.004
Abstract: Environmental teratogens such as smoking are known risk factors for developmental disorders such as cleft palate. While smoking rates have declined, a new type of smoking, called vaping is on the rise. Vaping is the… read more here.
Keywords: xenopus embryos; craniofacial defects; acid signaling; embryos exposed ... See more keywords
Foxd4l1.1 negatively regulates transcription of neural repressor ventx1.1 during neuroectoderm formation in Xenopus embryos
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-73662-4
Abstract: Neuroectoderm formation is the first step in development of a proper nervous system for vertebrates. The developmental decision to form a non-neural ectoderm versus a neural one involves the regulation of BMP signaling, first reported… read more here.
Keywords: neuroectoderm formation; xenopus embryos; neural repressor; repressor ventx1 ... See more keywords
Dusp1 modulates activin/smad2 mediated germ layer specification via FGF signal inhibition in Xenopus embryos
Sign Up to like & getrecommendations! Published in 2020 at "Animal Cells and Systems"
DOI: 10.1080/19768354.2020.1847732
Abstract: ABSTRACT Activin, a member of the transforming growth factor (TGF-β) superfamily, induces mesoderm, endoderm and neuro-ectoderm formation in Xenopus embryos. Despite several previous studies, the complicated gene regulatory network and genes involved in this induction… read more here.
Keywords: dusp1; xenopus embryos; activin smad2; expression ... See more keywords
In Vivo Assessment of Drug-Induced Hepatotoxicity Using Xenopus Embryos.
Sign Up to like & getrecommendations! Published in 2020 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot106096
Abstract: Failure to predict drug-induced toxicity reactions is a major problem contributing to a high attrition rate and tremendous cost in drug development. Drug screening in X. laevis embryos is high-throughput relative to screening in rodents,… read more here.
Keywords: induced hepatotoxicity; xenopus embryos; drug induced; drug ... See more keywords
Chambers for Culturing and Immobilizing Xenopus Embryos and Organotypic Explants for Live Imaging.
Sign Up to like & getrecommendations! Published in 2021 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot107649
Abstract: Live imaging of Xenopus embryos and organotypic explants can be challenging because of their large size and slippery nature. This protocol covers the preparation of special chambers for immobilizing Xenopus embryos and embryonic explants for… read more here.
Keywords: embryos; embryos organotypic; xenopus embryos; organotypic explants ... See more keywords
Genomics Methods for Xenopus Embryos and Tissues.
Sign Up to like & getrecommendations! Published in 2020 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.top097915
Abstract: High-throughput sequencing methods have created exciting opportunities to explore the regulatory landscape of the entire genome. Here we introduce methods to characterize the genomic locations of bound proteins, open chromatin, and sites of DNA-DNA contact… read more here.
Keywords: xenopus embryos; methods xenopus; genomics methods; embryos tissues ... See more keywords
Transcriptomics and Proteomics Methods for Xenopus Embryos and Tissues.
Sign Up to like & getrecommendations! Published in 2019 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.top098350
Abstract: The general field of quantitative biology has advanced significantly on the back of recent improvements in both sequencing technology and proteomics methods. The development of high-throughput, short-read sequencing has revolutionized RNA-based expression studies, while improvements… read more here.
Keywords: transcriptomics proteomics; xenopus embryos; proteomics methods; methods xenopus ... See more keywords