P2X Electrophysiology and Surface Trafficking in Xenopus Oocytes.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9717-6_18
Abstract: Xenopus oocytes serve as a standard heterologous expression system for the study of various ligand-gated ion channels including ATP P2X receptors. Here we describe the whole-cell two-electrode voltage clamp and biotinylation/Western blotting techniques to investigate… read more here.
Keywords: p2x electrophysiology; electrophysiology surface; surface trafficking; xenopus oocytes ... See more keywords
Inositol 1, 4, 5-trisphosphate receptor is required for spindle assembly in Xenopus oocytes
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Biology of the Cell"
DOI: 10.1091/mbc.e22-06-0218
Abstract: The extent to which calcium signaling participates in specific events of animal cell meiosis or mitosis is a subject of enduring controversy. We have previously demonstrated that buffering intracellular calcium with 1,2-bis(2-aminophenoxy)ethane-N,N,N’,N’-tetraacetic acid (BAPTA, a… read more here.
Keywords: calcium; inositol trisphosphate; spindle assembly; xenopus oocytes ... See more keywords
Patch-Clamp and Perfusion Techniques to Study Ion Channels Expressed in Xenopus Oocytes.
Sign Up to like & getrecommendations! Published in 2018 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot099051
Abstract: The Xenopus oocyte expression system is ideal for electrophysiological characterization of voltage-dependent and ligand-dependent ion channels because of its relatively low background of endogenous channels and the large size of the cell. Here, we present… read more here.
Keywords: ion channels; xenopus oocytes; patch clamp; channels expressed ... See more keywords
Pyrophosphate Stimulates the Phosphate-Sodium Symporter of Trypanosoma brucei Acidocalcisomes and Saccharomyces cerevisiae Vacuoles
Sign Up to like & getrecommendations! Published in 2019 at "mSphere"
DOI: 10.1128/msphere.00045-19
Abstract: Acidocalcisomes, first described in trypanosomes and known to be present in a variety of cells, have similarities with S. cerevisiae vacuoles in their structure and composition. Both organelles share a Na+/Pi symporter involved in Pi… read more here.
Keywords: xenopus oocytes; saccharomyces cerevisiae; trypanosoma brucei; brucei acidocalcisomes ... See more keywords
Lithobates catesbeianus (American Bullfrog) oocytes: a novel heterologous expression system for aquaporins
Sign Up to like & getrecommendations! Published in 2018 at "Biology Open"
DOI: 10.1242/bio.031880
Abstract: ABSTRACT Xenopus laevis oocytes are a valuable tool for investigating the function of membrane proteins. However, regulations around the world, specifically in Brazil, render the import of Xenopus laevis frogs impractical, and, in some cases,… read more here.
Keywords: system; cys; xenopus oocytes; expression ... See more keywords
Microtransplantation of Postmortem Native Synaptic mGluRs Receptors into Xenopus Oocytes for Their Functional Analysis
Sign Up to like & getrecommendations! Published in 2022 at "Membranes"
DOI: 10.3390/membranes12100931
Abstract: Metabotropic glutamate receptors (mGluRs) are membrane receptors that play a central role in the modulation of synaptic transmission and neuronal excitability and whose dysregulation is implicated in diverse neurological disorders. Most current understanding about the… read more here.
Keywords: glutamate; microtransplantation postmortem; metabotropic; group ... See more keywords
Xenopus Oocytes as a Powerful Cellular Model to Study Foreign Fully-Processed Membrane Proteins
Sign Up to like & getrecommendations! Published in 2022 at "Membranes"
DOI: 10.3390/membranes12100986
Abstract: The use of Xenopus oocytes in electrophysiological and biophysical research constitutes a long and successful story, providing major advances to the knowledge of the function and modulation of membrane proteins, mostly receptors, ion channels, and… read more here.
Keywords: membrane proteins; fully processed; model; membrane ... See more keywords
Asbestos Fibers Enhance the TMEM16A Channel Activity in Xenopus Oocytes
Sign Up to like & getrecommendations! Published in 2023 at "Membranes"
DOI: 10.3390/membranes13020180
Abstract: Background: The interaction of asbestos fibers with target cell membranes is still poorly investigated. Here, we detected and characterized an enhancement of chloride conductance in Xenopus oocyte cell membranes induced by exposure to crocidolite (Croc)… read more here.
Keywords: cell; asbestos; membrane; xenopus oocytes ... See more keywords
Recording Sodium Self-Inhibition of Epithelial Sodium Channels Using Automated Electrophysiology in Xenopus Oocytes
Sign Up to like & getrecommendations! Published in 2023 at "Membranes"
DOI: 10.3390/membranes13050529
Abstract: The epithelial sodium channel (ENaC) is a key regulator of sodium homeostasis that contributes to blood pressure control. ENaC open probability is adjusted by extracellular sodium ions, a mechanism referred to as sodium self-inhibition (SSI).… read more here.
Keywords: self inhibition; sodium self; sodium; epithelial sodium ... See more keywords