Disruption of tp53 leads to cutaneous nevus and melanoma formation in Xenopus tropicalis
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Oncology"
DOI: 10.1002/1878-0261.13301
Abstract: In humans, germline TP53 mutations predispose carriers to a wide spectrum of cancers, which is known as Li–Fraumeni syndrome (LFS). To date, the association of melanomas with LFS remains unestablished. No melanomas have been reported… read more here.
Keywords: disruption; xenopus tropicalis; tp53; lfs ... See more keywords
Husbandry, General Care, and Transportation of Xenopus laevis and Xenopus tropicalis.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8784-9_1
Abstract: Maintenance of optimal conditions such as water parameters, diet, and feeding is essential to a healthy Xenopus laevis and Xenopus tropicalis colony and thus to the productivity of the lab. Our prior husbandry experience as… read more here.
Keywords: xenopus; laevis xenopus; xenopus laevis; xenopus tropicalis ... See more keywords
CRISPR/Cas9 F0 Screening of Congenital Heart Disease Genes in Xenopus tropicalis.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8784-9_12
Abstract: In the US and Europe, birth defects are the leading cause of infant mortality. Among birth defects, Congenital Heart Disease (CHD) occurs in approximately 8 out of 1000 live births, affects 1.3 million newborns per… read more here.
Keywords: crispr cas9; heart disease; disease; xenopus tropicalis ... See more keywords
Construction and characterization of a BAC library for functional genomics in Xenopus tropicalis.
Sign Up to like & getrecommendations! Published in 2017 at "Developmental biology"
DOI: 10.1016/j.ydbio.2016.05.015
Abstract: Large insert genomic DNA libraries are useful resources for genomic studies. Although the genome of Xenopus tropicalis stands as the amphibian reference genome because it benefitted from large-scale sequencing studies, physical mapping resources such as… read more here.
Keywords: bac library; bac; construction characterization; characterization bac ... See more keywords
Three-dimensional folding dynamics of the Xenopus tropicalis genome
Sign Up to like & getrecommendations! Published in 2021 at "Nature Genetics"
DOI: 10.1038/s41588-021-00878-z
Abstract: Animal interphase chromosomes are organized into topologically associating domains (TADs). How TADs are formed is not fully understood. Here, we combined high-throughput chromosome conformation capture and gene silencing to obtain insights into TAD dynamics in… read more here.
Keywords: three dimensional; folding dynamics; xenopus tropicalis; dynamics xenopus ... See more keywords
Obtaining Xenopus tropicalis Eggs.
Sign Up to like & getrecommendations! Published in 2021 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot106344
Abstract: Xenopus is a powerful model system for cell and developmental biology in part because frogs produce thousands of eggs and embryos year-round. For cell biological studies, egg extracts can mimic many processes in a cell-free… read more here.
Keywords: xenopus; biology; xenopus tropicalis; obtaining xenopus ... See more keywords
CRISPR-Cas9 Mutagenesis in Xenopus tropicalis for Phenotypic Analyses in the F0 Generation and Beyond.
Sign Up to like & getrecommendations! Published in 2021 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot106971
Abstract: CRISPR-Cas9 mutagenesis is being widely used to create targeted loss-of-function mutations in the diploid frog Xenopus tropicalis Here we describe a simple mutagenesis protocol using microinjection of Cas9 protein or mRNA, together with synthetic guide… read more here.
Keywords: cas9 mutagenesis; crispr cas9; xenopus tropicalis; mutagenesis ... See more keywords
Best Practices for Xenopus tropicalis Husbandry.
Sign Up to like & getrecommendations! Published in 2022 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.top106252
Abstract: Xenopus tropicalis has been adopted by laboratories as a developmental genetic system because of its diploid genome and short generation time, contrasting with Xenopus laevis, which is allotetraploid and takes longer to reach sexual maturity.… read more here.
Keywords: tropicalis husbandry; best practices; xenopus tropicalis; practices xenopus ... See more keywords
Cardiac telocytes exist in the adult Xenopus tropicalis heart
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Cellular and Molecular Medicine"
DOI: 10.1111/jcmm.14947
Abstract: Recent research has revealed that cardiac telocytes (CTs) play an important role in cardiac physiopathology and the regeneration of injured myocardium. Recently, we reported that the adult Xenopus tropicalis heart can regenerate perfectly in a… read more here.
Keywords: tropicalis heart; adult xenopus; telocytes exist; heart ... See more keywords
Epithelial-Mesenchymal Transition Promotes the Differentiation Potential of Xenopus tropicalis Immature Sertoli Cells
Sign Up to like & getrecommendations! Published in 2019 at "Stem Cells International"
DOI: 10.1155/2019/8387478
Abstract: Epithelial-mesenchymal transition (EMT) is a fundamental process in embryonic development by which sessile epithelial cells are converted into migratory mesenchymal cells. Our laboratory has been successful in the establishment of Xenopus tropicalis immature Sertoli cells… read more here.
Keywords: tropicalis immature; mesenchymal transition; epithelial mesenchymal; differentiation potential ... See more keywords
Functional analysis of thyroid hormone receptor beta in Xenopus tropicalis founders using CRISPR-Cas
Sign Up to like & getrecommendations! Published in 2018 at "Biology Open"
DOI: 10.1242/bio.030338
Abstract: ABSTRACT Amphibians provide an ideal model to study the actions of thyroid hormone (TH) in animal development because TH signaling via two TH receptors, TRα and TRβ, is indispensable for amphibian metamorphosis. However, specific roles… read more here.
Keywords: crispr cas; xenopus tropicalis; metamorphosis; using crispr ... See more keywords