A Strong Hybrid Fatty Acid Inducible Transcriptional Sensor Built From Yarrowia lipolytica Upstream Activating and Regulatory Sequences
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology Journal"
DOI: 10.1002/biot.201700248
Abstract: The engineering of Yarrowia lipolytica to accumulate lipids with high titers and productivities has been enabled with a handful of constitutive promoters for pathway engineering. However, the development of promoters that are both strong and… read more here.
Keywords: hybrid fatty; fatty acid; strong hybrid; acid inducible ... See more keywords
CRISPRi repression of nonhomologous end‐joining for enhanced genome engineering via homologous recombination in Yarrowia lipolytica
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26404
Abstract: In many organisms of biotechnological importance precise genome editing is limited by inherently low homologous recombination (HR) efficiencies. A number of strategies exist to increase the effectiveness of this native DNA repair pathway; however, most… read more here.
Keywords: homologous recombination; repression; nonhomologous end; crispri ... See more keywords
Genome‐wide CRISPR‐Cas9 screen reveals a persistent null‐hyphal phenotype that maintains high carotenoid production in Yarrowia lipolytica
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28219
Abstract: Yarrowia lipolytica is a metabolic engineering host of growing industrial interest due to its ability to metabolize hydrocarbons, fatty acids, glycerol, and other renewable carbon sources. This dimorphic yeast undergoes a stress‐induced transition to a… read more here.
Keywords: screen; genome wide; yarrowia lipolytica; production ... See more keywords
Impact of oxygen availability on heterologous geneexpression and polypeptide secretion dynamics in Yarrowia lipolytica‐based protein production platforms
Sign Up to like & getrecommendations! Published in 2020 at "Yeast"
DOI: 10.1002/yea.3499
Abstract: Industrially relevant traits of Yarrowia lipolytica, like high growth rate, capacity to grow at high cell density or to synthesize biomolecules with high productivities, strongly rely on sufficient oxygen provision. Although the impact of oxygen… read more here.
Keywords: secretion; yarrowia lipolytica; impact oxygen; rprot synthesis ... See more keywords
Insights into the glycerol transport of Yarrowia lipolytica.
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DOI: 10.1002/yea.3702
Abstract: Cellular membranes separate cells from the environment and hence, from molecules essential for their survival. To overcome this hurdle, cells developed specialized transport proteins for the transfer of metabolites across these membranes. Crucial metabolites that… read more here.
Keywords: glycerol transport; transport; glycerol; yarrowia lipolytica ... See more keywords
Methods to Activate and Elucidate Complex Endogenous Sugar Metabolism in Yarrowia lipolytica.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1414-3_12
Abstract: Yarrowia lipolytica has endogenous metabolism to use complex sugars derived from lignocellulosic biomass. However, many of these pathways are cryptic and hence either inactive or inefficient for xylose, arabinose, and cellobiose assimilation. Here we present… read more here.
Keywords: activate elucidate; metabolism; methods activate; endogenous sugar ... See more keywords
Droplet-Based Microfluidic High-Throughput Screening of Enzyme Mutant Libraries Secreted by Yarrowia lipolytica.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1414-3_14
Abstract: Yarrowia lipolytica has emerged as an attractive solution for screening enzyme activities thanks to the numerous tools available for heterologous protein production and its strong secretory ability. Nowadays, activity screening for improved enzymes mostly relies… read more here.
Keywords: droplet; droplet based; screening enzyme; based microfluidic ... See more keywords
CRISPR Interference and Activation to Modulate Transcription in Yarrowia lipolytica.
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DOI: 10.1007/978-1-0716-1414-3_6
Abstract: Recent developments in RNA-guided nuclease technologies have advanced the engineering of a wide range of organisms, including the nonconventional yeast Yarrowia lipolytica. Y. lipolytica has been the focus of a range of synthetic biology and… read more here.
Keywords: transcription; crispr interference; biology; activation ... See more keywords
Implementing CRISPR-Cas12a for Efficient Genome Editing in Yarrowia lipolytica.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1414-3_7
Abstract: The oleaginous yeast Yarrowia lipolytica has emerged as an industrially relevant chassis to produce various valuable chemicals. Metabolic engineering of Y. lipolytica relies on the availability of genetic engineering tools. Existing engineering strategies for this yeast… read more here.
Keywords: genome editing; crispr cas12a; efficient genome; lipolytica ... See more keywords
Selection of Heterologous Protein-Producing Strains in Yarrowia lipolytica.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9024-5_6
Abstract: Yarrowia lipolytica has emerged as an alternative expression system for heterologous protein production and enzyme evolution. Several different expression systems dedicated for this species have been developed, ranging from the simple cloning of expression vectors… read more here.
Keywords: producing strains; selection; expression; heterologous protein ... See more keywords
Erratum to: Identification and characterization of EYK1, a key gene for erythritol catabolism in Yarrowia lipolytica
Sign Up to like & getrecommendations! Published in 2017 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-017-8412-4
Abstract: Multiple alignments of YALY0F01606g genes with the DAK1, DAK2 and DAK3 in the lipolytica clade. Homologs of EYK1 in Yarrowia species were identified by Blast on the private site of GRYC using EYK1 gene as… read more here.
Keywords: yarrowia; erratum identification; gene; identification characterization ... See more keywords