Purification of Yeast Artificial Chromosome DNA for Microinjection Using a Two-Gel Electrophoresis Procedure.
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DOI: 10.1101/pdb.prot093930
Abstract: This protocol describes a method to purify yeast artificial chromosome (YAC) DNA for microinjection. YAC DNA solutions cannot be concentrated by standard DNA precipitation methods and resuspended into smaller volumes. Attempts to precipitate YAC DNA… read more here.
Keywords: yeast artificial; gel electrophoresis; dna; yac dna ... See more keywords
Large-Scale Preparation of Yeast Agarose Plugs to Isolate Yeast Artificial Chromosome DNA.
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DOI: 10.1101/pdb.prot093955
Abstract: The microinjection of DNA directly into the pronuclei of fertilized zygotes is the most extensively used method of gene transfer in the mouse. The injection of very large pieces of DNA, including bacterial artificial chromosomes… read more here.
Keywords: large scale; yeast artificial; dna; yeast agarose ... See more keywords
Evidence for yeast artificial synthesis in SARS-CoV-2 and SARS-CoV-1 genomic sequences
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DOI: 10.12688/f1000research.72956.4
Abstract: Background:Â Knowledge about the origin of SARS-CoV-2 is necessary for both a biological and epidemiological understanding of the COVID-19 pandemic. Evidence suggests that a proximal evolutionary ancestor of SARS-CoV-2 belongs to the bat coronavirus family. However,… read more here.
Keywords: sars cov; cov sars; evidence yeast; yeast artificial ... See more keywords